Decreased hemolysis and lipid peroxidation in blood during storage in the presence of nicotinic acid.

Author(s): Arun P, Padmakumaran Nair KG, Manojkumar V, Deepadevi KV, Lakshmi LR, Kurup PA

Publication: Vox Sang, 1999, Vol. 76, Page 220-5

PubMed ID: 10394141 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the values of several indicators of lipid peroxidation, antioxidant protection, and RBC lysis, as well as to determine the pH level and amount of DEHP leaching into whole blood stored in DEHP plasticized bags after the addition of various concentrations of nicotinic acid (3.0, 4.5, or 6.8 mg/100 mg blood) to the usual CPDA storage solution. Values were obtained from plasma treated with the anticoagulant citrate-phosphate-dextrose-adenine after whole blood refrigeration at 4 degrees C for several weeks.

   Summary of Findings:

   Plasma hemoglobin, potassium, and lactate dehydrogenase levels were found to decrease when nicotinic acid was added to the CPDA solution. There was also less of an increase in the levels of malondialdehyde (MDA), conjugated dienes, and DEHP leaching as storage duration increased compared with blood stored in CPDA solution alone. The levels of reduced glutathione, vitamin E, and 2,3-diphosphoglycerate increased when nicotinic acid was added, and the recovery of normal shape of RBCs was also improved. Nicotinic acid helped stored blood maintain a pH closer to 7.0 while levels of ATP, lactate, and glucose were not significantly different than in CPDA solution alone. Overall, the addition of nicotinic acid was beneficial in decreasing RBC lysis, lipid peroxidation, and leaching of DEHP into the blood while increasing antioxidant protection. The magnitude of these effects was propotional to the concentration of nicotinic acid in stored specimens.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Colorimetric assay
   Protein	Spectrophotometry
   Small molecule	Colorimetric assay
   Small molecule	Chemiluminescence
   Small molecule	Spectrophotometry
   Small molecule	HPLC
   Electrolyte/Metal	pH

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 d 28 d 42 d
   Storage	Type of storage container	DEHP plasticized bags
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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