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Studies on the mechanism of human red cell loss of viability during storage at +4 degrees C. II. Relation between cellular morphology and viability.

Author(s): Högman CF, de Verdier CH, Borgström L

Publication: Vox Sang, 1987, Vol. 52, Page 20-3

PubMed ID: 3604164 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of warming red blood cells (RBCs) at 37 degrees C for 1 h after 42 days refrigerated at 4 degrees C and chromium labeling on morphology, fluidity and adenylate content. Blood was collected in citrate phosphate dextrose (CPD)/saline adenine glucose mannitol (SAGM). After centrifugation, additional SAGM was added to RBCs for storage at 4 degrees C for 42 days. After storage, a subset of specimens were warmed to 37 degrees C for 1 h. Specimens were assayed before and after chromium labeling.

   Summary of Findings:

   Compared to when RBCs were not warmed, warming of the RBCs for 1 h led to a significantly higher percentage of cells with discocyte or echinocyte morphology, both when assayed before (51% versus 39%, p<0.001) and after chromium-labeling (60% versus 46%, p<0.01). Warming RBCs also increased the fluidity before chromium-labeling (86 versus 71 Pa/S, p<0.02), but the effect of warming on fluidity was not assessed after chromium labeling. ATP and total adenine nucleotides were lower in specimens warmed for 1 h than in specimens that were never warmed when assayed before chromium-labeling (p<0.05, both), but warming had no effects on ATP or total adenine nucleotides when assayed after chromium labeling. The percentage of hemolyzed specimens, adenylate energy charge, glucose, lactate and potassium levels were unaffected by warming the cells for 1 h. The authors postulate that the beneficial effect of warming is short-term, as they did not find an increase in post-transfusion survival of RBCs.

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	4 degrees C 37 degrees C
   Biospecimen Aliquots and Components	Biospecimen components	Chromium-labeled cells Unlabeled cells

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