Short-term stability of the molecular forms of prostate-specific antigen and effect on percent complexed prostate-specific antigen and percent free prostate-specific antigen.

Author(s): Sokoll LJ, Bruzek DJ, Dua R, Dunn W, Mohr P, Wallerson G, Eisenberger M, Partin AW, Chan DW

Publication: Urology, 2002, Vol. 60, Page 24-30

PubMed ID: 12384159 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage temperature and duration on tPSA, fPSA, and cPSA in serum from healthy men and those with prostate cancer or elevated PSA in the absence of cancer using two analyzers. Blood was centrifuged within 2 h of collection, and serum was analyzed immediately or following experimental storage.

   Summary of Findings:

   tPSA, cPSA and fPSA were stable in serum stored for 1 week at -20 degrees C. tPSA decreased significantly between 48 h and 1 week of storage at 4 degrees C or room temperature using the Access analyzer (<10%) and using the Immuno I analyzer (<20%), but the changes were only clinically significant when storage was at room temperature. Similarly, cPSA declined significantly after more than 8 h of storage at room temperature or 24 h at 4 degrees C, but the cPSA to tPSA ratio was unaffected by storage at room temperature or -20 degrees C and increased insignificantly (10-15%) with storage at 4 degrees C for 1 week. fPSA declined significantly after storage at room temperature for more than 48 h (10% decline) or at 4 degrees C for more than 24 h (15% decline), but the percent fPSA of tPSA was unaffected by storage at room temperature and declined by 20% after 48 h of storage at 4 degrees C. fPSA levels were more variable than cPSA levels, and their variability increased with storage at 4 degrees C and room temperature.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal
   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Glycoprotein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 h 4 h 8 h 24 h 48 h 1 week
   Storage	Storage temperature	-20 degrees C 4 degrees C Room temperature
   Clinical chemistry/auto analyzer Specific	Technology platform	Beckman Coulter Access analyzer Bayer Immuno 1 analyzer

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