Whole blood treated with riboflavin and ultraviolet light: quality assessment of all blood components produced by the buffy coat method.
Author(s): Schubert P, Culibrk B, Karwal S, Serrano K, Levin E, Bu D, Bhakta V, Sheffield WP, Goodrich RP, Devine DV
Publication: Transfusion, 2015, Vol. 55(4), Page 815-23
PubMed ID: 25355434 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to compare storage properties of red blood cells (RBCs), plasma and platelets (Plts) from untreated blood with those obtained from blood that was Mirasol-treated.
Conclusion of Paper
When whole blood was Mirasol (ultraviolet and riboflavin)-treated before separation of the Plts, RBC and plasma by the buffy-coat method, the resultant Plts were less affected by storage than Plts treated after separation. In contrast, RBCs from Mirasol-treated blood showed faster RBC deterioration with storage, and plasma from Mirasol-treated blood had reduced coagulation factors and prolonged clotting times compared to those from untreated blood.
Studies
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Study Purpose
The purpose of this study was to determine the effect of Mirasol treatment of whole blood versus Plts and storage duration on markers of Plt quality. The experiment was conducted 6 times. Each experiment involved pooling 8 whole blood units which were split and treated with Mirasol or not, and then held overnight before being processed by the buffy-coat method into plasma, RBCs and Plts. Plts from untreated blood were Mirasol-treated, and stored along with those from Mirasol-treated blood in illumination bags at 20-24°C with constant agitation for up to 7 days.
Summary of Findings:
With increasing storage duration, glucose levels, extent of shape change (ESC), and response to Plt activation declined, and lactate levels, Plt activation, and the percentage of Plts expressing annexin V increased, but the changes tended to be smaller when whole blood rather than the Plts was treated with Mirasol. When blood rather than Plts was treated with Mirasol, lactate levels were higher on day 1 and 2 (p<0.05), ESC was higher on day 2 and 7 (p<0.05, both), the response to Plt activation was higher on days 2, 5 and 7 (p<0.05, all), pH and glucose levels were higher and the percentage of Annexin V positive Plts was lower on day 5 (p<0.05, all) and 7 (p<0.05, all), and there was lower Plt activation on day 7 (p<0.05). There was no effect of the timing of Mirasol treatment on Plt concentration, or mean Plt volume (MPV).
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Cell count/volume Flow cytometry Electrolyte/Metal Clinical chemistry/auto analyzer Morphology Flow cytometry Gas Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Carbohydrate Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 1 day
2 days
5 days
7 days
Biospecimen Aliquots and Components Blood processing method Mirasol treatment of whole blood
Mirasol treatment of platelets
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Study Purpose
The purpose of this study was to determine the effects of treating whole blood with Mirasol and subsequent storage on markers of RBC quality. The experiment was conducted 6 times. Each experiment involved pooling 8 whole blood units which were split and treated or not, and then held overnight before being processed by the buffy coat method into plasma, RBCs and Plts. RBCs were stored at 4°C for up to 6 weeks.
Summary of Findings:
Over the 6 weeks of refrigerated storage, pH and glucose and adenosine triphosphate (ATP) levels declined, and hemolysis, potassium levels, and microparticle counts increased, and the effect of storage was generally larger in RBCs from Mirasol-treated blood than untreated blood. When whole blood was treated with Mirasol, the resultant RBCs had lower levels of potassium and ATP at all timepoints (p<0.05, all), higher hemolysis after 1 week or more of refrigerated storage (p<0.05), and higher mean corpuscular volume (MCV) and microparticle counts after 3 weeks or more of refrigerated storage (p<0.05). RBC pH, levels of glucose and lactate, and RBC volume were unaffected by Mirasol treatment of whole blood.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Cell count/volume Hematology/ auto analyzer Cell count/volume Flow cytometry Electrolyte/Metal Clinical chemistry/auto analyzer Protein ELISA Small molecule Clinical chemistry/auto analyzer Small molecule HPLC Morphology Flow cytometry Protein Hematology/ auto analyzer Gas Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 days
1 week
3 weeks
6 weeks
Biospecimen Aliquots and Components Blood processing method Mirasol-treated whole blood
Untreated whole blood
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Study Purpose
The purpose of this study was to determine the effects of treating whole blood with Mirasol on markers of plasma quality. The experiment was conducted 6 times. Each experiment involved pooling 8 whole blood units which were split and treated or not, and the held overnight before being processed by the buffy-coat method. Plasma was immediately frozen at -80°C and shipped (total 3-5 months storage) before thawing at 37°C for analysis.
Summary of Findings:
Compared to plasma from untreated blood, plasma from Mirasol-treated blood had lower Factor (F)V (-26%, p<0.05), FVII (-24%, p<0.05), FVIII (-43%, p<0.05), protein S (-13%, p<0.05), α2-antiplasmin (-10%, p<0.05), and fibrinogen (-29%, p<0.05) levels and longer prothrombin time (PT, 15%, p<0.05) and activated partial thromboplastin time (APTT, 26%, p<0.05).
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Hematology/ auto analyzer Morphology Hematology/ auto analyzer Glycoprotein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood processing method Mirasol-treated whole blood
Untreated whole blood