Relocation of cryopreserved umbilical cord blood samples using a high-capacity dry shipper to a new laboratory: a cord blood banking experience.
Author(s): Thiagarajah K, Wong CY, Vijayan VV, Ooi GC, Ng MT, Cheong SK, Then KY
Publication: Transfusion, 2015, Vol. 55, Page 1028-32
PubMed ID: 25472857 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of transport in a high capacity dry shipper on umbilical cord blood (UCB) quality.
Conclusion of Paper
Total nucleated cell (TNC) counts, cell viability and the percentage of CD34 positive cells were not affected by transport of UCB in a dry shipper.
Studies
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Study Purpose
The purpose of this study was to determine the effects of transport in a high capacity dry shipper on UCB TNC counts, viability and the percentage of CD34 positive cells. UCB from 3 donors was processed by the hetastarch method, cryopreserved, aliquoted into 24 vials/donor and stored in 12 liquid nitrogen freezers. One specimen from each donor and each freezer was thawed and analyzed. The next day, a specimen from each donor and each freezer was transferred to the dry shipper which maintained a temperature below -150°C. The dry shippers were moved 4.2 km by truck at low speed. Upon arrival, specimens were held overnight in dry shippers or temporary liquid nitrogen freezers before transferring into the cooled original liquid nitrogen freezers, until thawing and analysis.
Summary of Findings:
TNC counts, cell viability and the percentage of CD34 positive cells were not affected by transport of UCB in a dry shipper.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Pregnant
Platform:
Analyte Technology Platform Cell count/volume Flow cytometry Cell count/volume Light microscopy Cell count/volume Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Between site transportation method Car/Truck
Not transported