Red blood cell storage in additive solution-7 preserves energy and redox metabolism: a metabolomics approach.
Author(s): D'Alessandro A, Nemkov T, Hansen KC, Szczepiorkowski ZM, Dumont LJ
Publication: Transfusion, 2015, Vol. 55, Page 2955-66
PubMed ID: 26271632 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to compare the metabolome of red blood cells (RBCs) stored in two different additive solutions (AS), AS-3 and AS-7.
Conclusion of Paper
Storage resulted in decreases in glutathione (GSH)/Redox and pentose phosphate pathway (PPP) metabolites and altered abundance of amino acids, purines, and fatty acids, regardless of storage solution; however, RBCs stored in AS-7 had higher levels of glycolytic and PPP intermediates and larger GSH pools. Further, , RBCs stored in AS-7 had lower levels of glucose, orthophosphate, and citrate and higher levels of mannitol and adenine, although the authors note this was expected based on the composition of the two storage solutions.
Studies
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Study Purpose
The purpose of this study was to compare the metabolome of RBCs stored in two different additive solutions, AS-3 and AS-7. RBCs were collected from 10 healthy donors by apheresis into ontaining citrate phosphate double dextrose (CP2D) or citrate phosphate dextrose (CPD). AS-3 was then added to CP2D RBCs, while AS-7 was added to CPD RBCs. All RBCs were leukoreduced by filtration and stored at 1-6˚C within 8 h of collection. On days 0, 7 and 42 aliquots were removed, frozen at -80˚C and shipped on dry ice for analysis. After arrival, metabolites were extracted and stored as supernatants at -80˚C until analysis by ultra high-performance liquid chromatography (UPLC) mass spectrometry (MS).
Summary of Findings:
A total of 229 metabolites were identified in RBC extracts. Partially supervised partial least square discriminant analysis (PLS-DA) discriminated between the two storage solutions and storage duration. Storage resulted in decreased GSH/Redox and PPP metabolites as well as altered abundance of amino acids, purines, and fatty acids, regardless of storage solution; however, RBCs stored in AS-7 had higher levels of glycolytic and PPP intermediates and larger GSH pools. These differences were particularly evident on day 7 of refrigerated storage. AS-7 stored RBCs had lower levels of glucose, orthophosphate, and citrate and higher levels of mannitol and adenine, which the authors note was expected based on the composition of the two storage solutions. Hematocrit (spun) was lower when RBCs were stored in AS-3 than in AS-7, which is attributable to the lower osmality of AS-3. A corresponding difference was not observed in MCV, most likely due to dilution before analysis.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Small molecule HPLC-MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 days
7 days
42 days
Storage Short-term storage solution AS-3
AS-7