Red blood cell storage affects the stability of cytosolic native protein complexes.
Author(s): Pallotta V, Rinalducci S, Zolla L
Publication: Transfusion, 2015, Vol. 55(8), Page 1927-36
PubMed ID: 25808351 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of refrigerated storage of red blood cells (RBCs) on cytosolic multiprotein complexes.
Conclusion of Paper
Refrigerated storage significantly affected 162 protein spots. Many of the associated proteins were involved in energy and redox metabolism.
Studies
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Study Purpose
The purpose of this study was to determine the effects of refrigerated storage of RBCs on cytosolic multiprotein complexes. Leukofiltered RBCs in saline-adenine-glucose-mannitol (SAGM) from 3 healthy male patients were stored at 4 degrees C. Cytosolic protein fractions were obtained through electrophoresis and separated by 1D and 2D PAGE. Proteins were identified using LC-MS/MS.
Summary of Findings:
A total of 162 protein spots were differentially affected by refrigerated storage duration in the 3 cytosolic protein fractions (84, 54 and 24 spots). LC-MS/MS revealed that many of the associated proteins were involved in energy and redox metabolism, as the authors expected, due to the decrease in glycolytic flux and increase in oxidative stress.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Protein LC-MS or LC-MS/MS Protein 1D/2D gels Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 days
21 days
35 days