Metabolomics profile comparisons of irradiated and nonirradiated stored donor red blood cells.
Author(s): Patel RM, Roback JD, Uppal K, Yu T, Jones DP, Josephson CD
Publication: Transfusion, 2015, Vol. 55, Page 544-52
PubMed ID: 25330719 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of irradiation on the metabolomic profile of red blood cells (RBCs) during refrigerated storage.
Conclusion of Paper
Based on m/z feature, RBCs were found to group according to refrigerated storage duration, with those stored for 2-7 days grouping together and specimens stored for ≥10 days grouping by whether or not they were subjected to irradiation. The arachidonic acid metabolism, linolenic acid metabolism, steroid biosynthesis, α-linolenic acid metabolism and glutathione pathways were implicated as differentially activated pathways during refrigerated storage and/or after irradiation of RBCs.
Studies
-
Study Purpose
The purpose of this study was to determine the effects of gamma irradiation and refrigerated storage on the metabolomic profile of RBCs. Citrate phosphate dextrose adenine solution 1 (CPDA-1) RBCs from 6 healthy donors were split on the day of collection, and one bag from each pair was exposed to 25 Gray (Gy) gamma irradiation while the other was left non-irradiated. RBCs were stored at 2-6°C on a rotating platform. At each timepoint, 5 1 mL aliquots were taken from each bag, snap-frozen and stored at -80°C until analysis.
Summary of Findings:
A total of 11,615 m/z features were identified, and by combining the top 200 features affected by storage duration, irradiation and the interaction between storage and irradiation a list of 599 m/z features was produced. These 599 m/z features formed distinct clusters based on treatment, with RBCs stored 2-7 days forming a distinct group, regardless of irradiation, and then closer groupings (smaller changes) with specimens stored for ≥10 days without irradiation and with irradiation. Using hierarchical clustering, 12 metabolite groups were identified. These clusters were found to distinguish between specimens stored for 2-7 days and those stored for ≥10 days, to distinguish based on specimen irradiation and to distinguish based on irradiation at longer durations. The arachidonic acid metabolism, linolenic acid metabolism, steroid biosynthesis, and α-linolenic acid metabolism pathways were identified, with a false discovery rate >0.05, as affected by storage and/or irradiation. Importantly, 12 of the 599 m/z features chosen corresponded to 5 metabolites in the arachidonic acid pathway. Targeted searching found correlations between metabolites affected by storage and/or irradiation and glutathione synthesis.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule LC-MS or LC-MS/MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood processing method Not irradiated
Gamma irradiated
Storage Storage duration 2 days
3 days
7 days
10 days
14 days
17 days
21 days
28 days
35 days