Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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In vitro study of platelet function confirms the contribution of the ultraviolet B (UVB) radiation in the lesions observed in riboflavin/UVB-treated platelet concentrates.

Author(s): Abonnenc M, Sonego G, Crettaz D, Aliotta A, Prudent M, Tissot JD, Lion N

Publication: Transfusion, 2015, Vol. 55(9), Page 2219-30

PubMed ID: 25873361 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare the effects of storage on the functional properties of amotosalen and UVA-treated, riboflavin and UVB-treated, only UVB-treated, and untreated PCs. Whole blood from 90 donors was held overnight before processing into buffy coats. A total of 15 buffy coats were used to make each PC pool. PC pools were then split for treatment and storage with agitation at 22°C. 3-6 biological replicates of each treatment were performed. Day 0 was defined as the day of blood collection.

   Summary of Findings:

   During room temperature storage of PCs, pH, bicarbonate, pCO2, and glucose levels declined, and pO2, LDH, lactate, and the percentage of Plts expressing CD62P or annexin V increased, but the significance was dependent on the duration of storage and treatment. Untreated Plts showed the least changes in levels of all analytes during storage. Compared to untreated control Plts, Plts treated with amotosalen and UVA had higher LDH release, percentage of Plts expressing annexin V and PAC1 fluorescence at all time-points (p<0.05, all), slightly lower bicarbonate on days 5 (p<0.001) and 7 (p<0.001), slightly lower pCO2 on day 7 (p<0.001), and slightly higher pO2 on day 5 (p<0.001), but comparable pH, CD62P expression, JC-1 aggregation, hypotonic shock response (HSR) and levels of glucose and lactate throughout the storage period. When compared to untreated Plts at the same time-point, UVB-treated Plts had higher lactate and pO2, lower glucose, pH and bicarbonate on days 5 (p<0.001, all) and 7 (p<0.001, all), a higher percentage of CD62P and annexin V expressing Plts, and higher PAC-1 mean fluorescence on day 2 (p<0.05, p<0.05, and p<0.001, respectively) and day 7 (p<0.05, p<0.05, and p<0.001, respectively), reduced hypotonic shock response (HSR) on day 2 (p<0.001) and 7 (p<0.001), lower pCO2 and percentage of JC-1 aggregates on day 7 (p<0.001, and p<0.05, respectively), and higher adherence to fibrinogen on day 2 (p<0.05). With the exception of the higher pO2 levels on days 5 and 7, all differences observed between untreated and UVB-treated Plts were also observed between untreated Plts and those treated with UVB and riboflavin. In addition to the aforementioned differences, on day 2, UVB and riboflavin treated Plts had lower glucose (p<0.001), bicarbonate (p<0.001) and pH (p<0.01) and higher lactate (p<0.001) than untreated Plts.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Morphology	Flow cytometry
   Protein	Clinical chemistry/auto analyzer
   Gas	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	2 days 5 days 7 days
   Biospecimen Aliquots and Components	Blood processing method	Amotosalen and UVA-treatment Ribofllavin and UVB-treatment UVB-treatment Untreated

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