AS-7 improved in vitro quality of red blood cells prepared from whole blood held overnight at room temperature.
Author(s): Veale MF, Healey G, Sran A, Payne KA, Zia M, Sparrow RL
Publication: Transfusion, 2015, Vol. 55, Page 108-114
PubMed ID: 25039791 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects refrigerated storage of red blood cells (RBCs) in additive solution-7 (AS-7 or SOLX) versus saline-adenine-glucose-mannitol (SAGM) after delayed blood processing on markers of RBC quality.
Conclusion of Paper
During refrigerated storage of RBCs, pH, adenosine triphosphate (ATP) and 2,3 diphosphoglycerate (2,3 DPG) levels declined, and hemolysis, potassium and the number of glycophorin A (GPA) positive and annexin V binding microparticles (MPs) increased. The decrease in ATP was partially attenuated by storage in AS-7 as opposed to SAGM. In addition to having significantly higher ATP, after storage in AS-7, RBCs had significantly higher intracellular pH and 2,3 DPG than RBCs stored in SAGM. Further, RBCs stored in AS-7 had lower osmotic fragility and fewer GPA positive MP counts than RBCs stored in SAGM. Extracellular pH, potassium, hemolysis, and the number of annexin V binding MPs were not affected by storage solution. RBC adhesion to endothelial cells increased non-significantly over the storage period and was not affected by storage solution.
Studies
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Study Purpose
The purpose of this study was to determine the effects of up to 42 days of refrigerated storage of RBCs in AS-7 versus SAGM after delayed blood processing on markers of RBC quality. Whole blood from 16 healthy individuals was stored at 22°C for 22 h before filtration of RBCs into SAGM or AS-7. RBCs were then held at 2-6°C for experimental storage.
Summary of Findings:
AS-7 stored RBCs had significantly higher intracellular pH at all time-points (p<0.001, all) and higher levels of 2,3 DPG beginning after 7 days of storage (p<0.001, day 7; p<0.05, day 14-42) and ATP beginning after 14 days of storage (p<0.05) than RBCs in SAGM at the same time-points. Further, RBCs stored in AS-7 had lower osmotic fragility and fewer GPA positive MP counts than RBCs stored in SAGM, with significance beginning on day 7 (p<0.05) and day 42 (p<0.0001), respectively. Extracellular pH, potassium, hemolysis, and the number of annexin V binding MPs were not affected by storage solution. The osmotic fragility of RBCs declined during storage in AS-7 but not in SAGM. During storage of RBCs, pH, ATP and 2,3 DPG levels declined, and hemolysis, potassium and the number of GPA positive and annexin V binding MPs increased, but the decrease in ATP was partially attenuated by storage in AS-7 as opposed to SAGM. RBC adhesion to endothelial cells increased non-significantly over the storage period and was not affected by storage solution.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Cell count/volume Flow cytometry Cell count/volume Hematology/ auto analyzer Morphology Spectrophotometry Small molecule pH Electrolyte/Metal Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 1 day
7 days
14 days
21 days
28 days
35 days
42 days
Storage Short-term storage solution AS-7 (SOLX)
SAGM