The heritability of metabolite concentrations in stored human red blood cells.
Author(s): van 't Erve TJ, Wagner BA, Martin SM, Knudson CM, Blendowski R, Keaton M, Holt T, Hess JR, Buettner GR, Ryckman KK, Darbro BW, Murray JC, Raife TJ
Publication: Transfusion, 2014, Vol. 54, Page 2055-63
PubMed ID: 24601981 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of storage solution and duration and hereditability on levels of adenosine triphosphate (ATP) in red blood cells (RBCs) and the correlation between the levels of ATP and 213 metabolites.
Conclusion of Paper
ATP concentrations in RBCs increased very slightly after 14 days of storage in additive solution 3 (AS-3), but then levels decreased steadily, reaching 50% of initial levels by day 56. ATP concentrations in RBCs stored in citrate phosphate double dextrose (CP2D) declined steadily, reaching 25% of initial levels by day 56. Storage effects on the levels of ATP, adenosine diphosphate (ADP) and 26 out of the 213 other metabolites showed >50% hereditability. Levels of 5 metabolites were weakly to modestly correlated with ATP concentrations, and 31 others showed weak to modest negative correlations with ATP.
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage solution, storage duration and hereditability on levels of ATP in RBCs and the correlation between the levels of ATP and 213 metabolites. Blood specimens were collected from 18 sets of twins (13 monozygous, 5 dizygous). Leukoreduced RBCs were prepared and placed in AS-3. The tubing from the leukoreduction filter to the secondary bag contained CP2D and was kept as segments. It is assumed the RBCs were stored at 4 degrees C. After storage, the specimen was processed for ATP measurements, but the supernatant containing ATP was then stored at -80 degrees C until analysis.
Summary of Findings:
ATP concentrations in RBCs increased very slightly after 14 days of storage in AS-3, but then levels decreased steadily, reaching 50% of initial levels by day 56. ATP concentrations in RBCs stored in CP2D declined steadily, reaching 25% of initial levels by day 56. The hereditability of the change in ATP concentration after day 28 and 56 of storage were 53% and 77%, respectively when stored in AS-3 and 64% and 77%, respectively when stored in CP2D. Changes in the levels of ADP during storage were also highly heritable (73% after 28 days). Levels of 87 out of the 213 metabolites in RBCs showed >20% hereditability, and levels of 26 other metabolites in RBCs showed >50% hereditability. Levels of 5 metabolites were weakly to modestly correlated with ATP concentrations (p<0.05), and 31 others showed weak to modest inverse correlations with ATP levels (p<0.05).
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein GC-MS Protein LC-MS or LC-MS/MS Small molecule Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Patient genotype Monozygous twins
Dizygotic twins
Storage Storage duration 0 days
14 days
28 days
42 days
56 days
Storage Short-term storage solution AS-3
CP2D