The effects of rejuvenation during hypothermic storage on red blood cell membrane remodeling.
Author(s): Kurach JD, Almizraq R, Bicalho B, Acker JP, Holovati JL
Publication: Transfusion, 2014, Vol. 54, Page 1595-603
PubMed ID: 24224647 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of refrigerated storage duration and rejuvenation on markers of red blood cell (RBC) storage lesions.
Conclusion of Paper
With increasing refrigerated storage, hemolysis and RBC microparticle (RMP) count increased, regardless of rejuvenation treatment, but adenosine triphosphate (ATP) levels declined only in the phosphate buffered saline (PBS, sham)-treated or untreated RBCs. Storage duration did not significantly affect RBC or RMP expression of phosphatidylserine (PS) or CD47. RBCs treated with rejuvenation solution had lower levels of hemolysis and higher levels of ATP than sham-treated RBCs and higher levels of ATP than untreated RBCs. Treatment with rejuvenation solution had no effect on RMP count or RMP expression of PS or CD47. Rejuvenation on day 28 resulted in 34% lower sphingomyelin and 19% higher cholesterol on day 49 compared to sham-treated or untreated controls.
Studies
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Study Purpose
The purpose of this study was to determine the effects of refrigerated storage duration and rejuvenation on markers of RBC storage lesions. Leukoreduced RBC units in saline adenine glucose manitol (SAGM) were obtained by the buffy coat method from specimens from 21 patients. Chilled rejuvenation solution or phosphate-buffered saline (PBS) (sham) were added to RBCs on day 28, 35, or 42 or the RBCs were left untreated. All RBCs were stored at 1-6°C.
Summary of Findings:
With longer refrigerated storage, hemolysis and RMP counts increased (p<0.01 and p<0.05, respectively), regardless of rejuvenation treatment, but ATP levels declined (p<0.05) only in the sham-treated or untreated RBCs. Storage duration did not significantly affect RBC or RMP expression of PS or CD47, but nonsignificant increases in RMP PS expression and decreases in RBC CD47 expression were observed for some treatment time-points. RBCs treated with rejuvenation solution had lower levels of hemolysis and higher levels of ATP than sham-treated RBCs (p<0.01 and p<0.05, respectively) and higher levels of ATP (p<0.05) than untreated RBCs. Treatment with rejuvenation solution had no affect on RMP counts, or RMP expression of PS or CD47. Rejuvenation on day 28 resulted in 34% lower sphingomyelin and 19% higher cholesterol on day 49 compared to sham-treated or untreated controls.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Small molecule Spectrophotometry Protein Clinical chemistry/auto analyzer Cell count/volume Spectrophotometry Cell count/volume Flow cytometry Cell count/volume Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 2 days
28 days
35 days
42 days
49 days
Storage Short-term storage solution Rejuvenation solution treated
PBS treated
Untreated