Overnight, room temperature hold of whole blood followed by 42-day storage of red blood cells in additive solution-7.
Author(s): Dumont LJ, Cancelas JA, Maes LA, Rugg N, Whitley P, Herschel L, Siegal AH, Szczepiorkowski ZM, Hess JR, Zia M
Publication: Transfusion, 2014, Vol. 55(3), Page 485-90
PubMed ID: 25234026 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of delayed processing of blood, red blood cell (RBC) storage solution and refrigeration duration on markers of RBC quality.
Conclusion of Paper
Storage of blood in additive solution (AS)-7 before processing into RBCs affected pH, morphology scores and lactate levels. Once processed into RBCs, storage solution affected hematocrit (Hct), bicarbonate, and glucose, and refrigerated storage duration affected pH, bicarbonate, glucose, adenosine triphosphate (ATP), lactate and hemolysis rate.
Studies
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Study Purpose
The purpose of this study was to determine the effects of delayed processing of blood, RBC storage solution and refrigeration duration on markers of RBC quality. RBCs in AS-7 (also called SOLX) were obtained from blood stored 0-2 h, 6-8 h or 18-24 h using the LEUKOSEP HWB-600-XL filter, and RBCs in AS-1 were obtained from blood stored 6-8 h using the RZ2000 leukoreduction filter.
Summary of Findings:
Regardless of processing delays or storage solution, the RBC pH, bicarbonate, glucose and ATP levels decreased, and lactate and hemolysis rate increased with increased storage duration. Immediately after processing, Hct, bicarbonate and glucose were lower in RBCs in AS-1 than AS-7, but mean corpuscular volume (MCV) was comparable. The Hct in AS-1 units remained constant during storage but the Hct of RBCs in AS-7 declined with storage. On day 42 of refrigerated storage, RBCs from blood held overnight before processing into AS-7 had higher ATP levels and fewer RBC microvesicles than RBCs from blood held for 6-8 h before processing into AS-1. When stored overnight before processing into RBCs in AS-7, the pH was lower and lactate higher, but ATP, bicarbonate, and glucose levels were comparable to those of RBCs processed into AS-7 after 8h or less. Further, after refrigerated storage of RBC, morphology scores were lower in RBCs processed after 16-24 h than those processed after <8 h, but there was no effect on potassium leakage or RBC microvesicles, and the effect on pH was no longer noticeable.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Small molecule Clinical chemistry/auto analyzer Carbohydrate Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Morphology Hematology/ auto analyzer Cell count/volume Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 0-2 h
6-8 h
18-24 h
Biospecimen Aliquots and Components Blood processing method LEUKOSEP HWB-600-XL leukoreduction filter
RZ2000 leukoreduction filter
Storage Short-term storage solution AS-1
AS-7 (SOLX)
Storage Storage duration 0 days
42 days
56 days