Extended storage of buffy coat platelet concentrates in plasma or a platelet additive solution.
Author(s): Slichter SJ, Bolgiano D, Corson J, Jones MK, Christoffel T, Bailey SL, Pellham E
Publication: Transfusion, 2014, Vol. 54, Page 2283-91
PubMed ID: 24673482 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of storage duration and storage solution on markers of quality in buffy coat (BC) platelets (plts) and to compare these markers between BC plts and plt rich plasma (PRP).
Conclusion of Paper
Annexin V binding increased with increasing room temperature plt storage, regardless of storage solution. Glucose levels were lower in plts stored in plasmalyte than in plasma, but levels were relatively stable during storage. Plt counts, pH, mean plt volume (MPV), morphology scores, extent of shape change (ESC), and hypotonic shock response (HSR) were not affected by 5-7 days of plt storage at room temperature or by storage solution. After 6 days of storage, glucose levels were lower in plts from PRP than plts from BC, but otherwise, the authors report no differences between plts from PRP and those from BC. Recovery of plts increased with increasing morphology scores and ESC and decreasing pH.
Studies
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Study Purpose
The purpose of this study was to determine the effects of room temperature storage duration and storage solution on markers of quality in BC plts and to compare BC plts with PRP. Whole blood was stored at 22 degrees C for 22 ± 2 h before separation of BC plts in plasmalyte or plasma. Plts were stored at 22 degrees C with agitation. PRP plts were obtained by centrifugation after storage of blood at room temperature for 30 min. PRP was stored in 15% acid-citrate-dextrose solution A (ACD-A).
Summary of Findings:
Annexin V binding increased with increasing room temperature storage of plts, regardless of storage solution. Glucose levels were lower in plts stored in plasmalyte than in plasma (p<0.001), but levels were relatively stable during storage. Plt counts, pH, MPV, morphology scores, ESC, and HSR were not affected by 5-7 days of storage at room temperature or storage solution. After 6 days of storage, glucose levels were lower in plts from PRP than plts from BC (p<0.001), but otherwise, the authors report no differences between plts from PRP and those from BC. Recovery of plts increased with increasing morphology scores and ESC and decreasing pH.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Cell count/volume Flow cytometry Cell count/volume Hematology/ auto analyzer Morphology Flow cytometry Carbohydrate Clinical chemistry/auto analyzer Small molecule pH Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 1 day
5 days
6 days
7 days
8 days
Storage Short-term storage solution Plasma
65% Plasmalyte
80% Plasmalyte
ACD-A
Biospecimen Aliquots and Components Blood and blood products Platelet-rich plasma
Platelets