Comprehensive metabolomic study of platelets reveals the expression of discrete metabolic phenotypes during storage.
Author(s): Paglia G, Sigurjónsson OE, Rolfsson O, Valgeirsdottir S, Hansen MB, Brynjólfsson S, Gudmundsson S, Palsson BO
Publication: Transfusion, 2014, Vol. 54(11), Page 2911-23
PubMed ID: 24840017 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of storage of apheresis platelets (plts) on the plt metabolome.
Conclusion of Paper
The expression of CD62P and CD63 and plt counts increased during the first 4 days of plt storage and then plateaued until day 7, at which time CD62P and CD63 expression increased again. By liquid chromatography–mass spectrometry (LC/MS), a total of 49 metabolites were identified in the extracellular fluid (exometabolome) and 96 were identified in the plts (endometabolome). During storage, extracellular levels of glucose, citric acid and glutamine decreased significantly, and lactate, succinic acid, malic acid, hypoxanthinine, inosine and xanthine increased significantly. During the first day of storage, intracellular levels of glucose 6-P, hypoxanthine, adenosine monophosphate (AMP), adenosine diphosphate (ADP), adenosine triphosphate (ATP) and inosine monophosphate (IMP) increased significantly, but after the first day, levels of these metabolites decreased. During the first 3 days of storage intracellular levels of lactic acid, malic acid and citric acid increased significantly, but then subsequently decreased. Intracellular mitochondrial activity decreased significantly between days 1-3 and 6-10 but increased between days 3-5. Hierarchical cluster analysis (HCA) showed that after 4 days of storage, there was a shift in the metabolic phenotype from glycolysis, pentose phosphate pathway, and glutathione metabolism to activation of the tricarboxylic acid (TCA) cycle and increased purine metabolism.
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage of apheresis plts on the plt metbolome. Six apheresis plt units in T-sol were stored in a shaking incubator at 22 degrees C.
Summary of Findings:
CD62P and CD63 expression and plt counts increased during the first 4 days of storage and then plateaued until day 7, at which time CD62P and CD63 expression increased again. A total of 49 metabolites were identified by LC/MS in the extracellular fluid (exometabolome) and 96 were identified in the plts (endometabolome). During storage, extracellular levels of glucose, citric acid and glutamine decreased significantly, and lactate, succinic acid, malic acid, hypoxanthinine, inosine and xanthine increased significantly. During the first day of storage, intracellular levels of glucose 6-P, hypoxanthine, AMP, ADP, ATP and IMP increased significantly, but after the first day, levels of these decreased. During the first 3 days of storage, intracellular levels of lactic acid, malic acid and citric acid increased significantly but then subsequently decreased. Intracellular mitochondrial activity decreased significantly between days 1-3 and 6-10 but increased between days 3-5. HCA showed that after 4 days of storage, there was a shift in the metabolic phenotype from glycolysis, pentose phosphate pathway, and glutathione metabolism to activation of the TCA cycle and increased purine metabolism.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Cell count/volume Hematology/ auto analyzer Gas Clinical chemistry/auto analyzer Peptide LC-MS or LC-MS/MS Small molecule Clinical chemistry/auto analyzer Protein ELISA Protein Clinical chemistry/auto analyzer Protein Hematology/ auto analyzer Protein Spectrophotometry Electrolyte/Metal Clinical chemistry/auto analyzer Cell count/volume Flow cytometry Small molecule pH Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 0 days
1 day
3 days
4 days
5 days
6 days
7 days
10 days