Automated preparation of whole blood-derived platelets suspended in two different platelet additive solutions and stored for 7 days.

Author(s): Cid J, Magnano L, Molina P, Diaz-Ricart M, Martínez N, Maymó RM, Puig L, Lozano M, Escolar G, Galán AM

Publication: Transfusion, 2014, Vol. 54, Page 426-33

PubMed ID: 23721299 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood processor type, storage solution and storage at room temperature on markers of Plt quality in PC. Leukoreduced PC were obtained from whole blood 1 day after collection. Buffy coats were obtained from whole blood using the Atreus 2C+ and were held for~4 h before processing of the buffy coats to PC in PASII using the OrbiSac and or PC were obtained directly from whole blood using the Atreus 3C and stored in PASII or PASIIIM. Each PC consisted of Plts from 4-5 healthy donors, and 8 PC pools were analyzed for each processor/storage solution.

   Summary of Findings:

   Compared to PC obtained using the Atreus 2C/OrbiSac, PC obtained with the Atreus 3C had lower volume (p<0.01) and a higher Plt count/L (p<0.01), regardless of storage duration and solution. However, Plt count/unit was not affected by type of blood processor, storage duration or storage solution. During room temperature storage, MPC decreased in all specimens (p<0.05 day 7 versus day 1, all), and while significant differences between processors were not apparent on day 1 or 7, on day 5, PC obtained with the Atreus 3C had higher MPC than those obtained with Atreus 2C/OrbiSac, regardless of storage solution (p<0.05, both). During room temperature storage of PC, glucose decreased, and lactate levels increased, and on day 5 and 7, glucose levels were lower and lactate levels were higher in PC obtained using the Atreus 3C and stored in Plt additive solution (PAS)-II than in PC obtained using the Atreus 2C/OrbiSac and stored in PAS-II or obtained by the Atreus 3C and stored in PAS-IIIM. The pH was higher in PC obtained using the Atreus 3C and stored in PAS-IIIM on day 1 than in PC obtained using the Atreus 3C and stored in PAS-II and on day 5 and 7 than in PC obtained using the Atreus 2C/OrbiSac and stored in PAS-II. Expression of Plt activation markers was not affected by storage duration, but was higher on day 5 and 7 in Plts obtained using the Atreus 3C and stored in PAS-IIIM than in Plts obtained using the Atreus 2C/OrbiSac and stored in PAS-II (p<0.05, both) or those obtained by the Atreus 3C and stored in PAS-II (p<0.05, both). Expression of GPIb, GPIIbIIA and GPIV, Factor V/Va, annexin V, and fibrinogen were not significantly affected by processor type, storage duration, or storage solution. vWF expression was lower on day 5 in Plts prepared with the Atreus 3C (either in PAS-II or IIIM) than in Plts prepared with the Atreus 2C in PAS-II. Processor type, storage solution and storage duration did not affect PO2 and PCO2 in PC.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Cell count/volume	Flow cytometry
   Cell count/volume	Hematology/ auto analyzer
   Gas	Clinical chemistry/auto analyzer
   Protein	Flow cytometry
   Glycoprotein	Flow cytometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	1 day 5 days 7 days
   Storage	Short-term storage solution	PAS-II PAS-IIIM
   Biospecimen Aliquots and Components	Blood processing method	Atreus 2C/OrbiSac Atreus3C

   View more