Additive solution-7 reduces the red blood cell cold storage lesion.
Author(s): Cancelas JA, Dumont LJ, Maes LA, Rugg N, Herschel L, Whitley PH, Szczepiokowski ZM, Siegel AH, Hess JR, Zia M
Publication: Transfusion, 2014, Vol. 55(3), Page 491-8
PubMed ID: 25233911 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effect of storage solution and processing delay on red blood cell (RBC) quality during refrigerated storage.
Conclusion of Paper
When blood was stored at room temperature for <2 h instead of <8 h before processing into RBCs in additive solution (AS)-7, lactate was higher immediately following processing, but no other significant effects were noted. Refrigerated storage in AS-7 partially attenuated the storage mediated decreases in pH, bicarbonate, adenosine triphosphate (ATP), morphology score and shed microvesicle protein and increases in hemolysis that were apparent with refrigerated storage in AS-1. However, storage in AS-7 resulted in larger decreases in glucose and larger increases in lactate than storage in AS-1.
Studies
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Study Purpose
The purpose of this study was to determine effect of collection locale, storage solution and processing delay on RBC quality during refrigerated storage. The study included 120 RBC units stored in AS-7 for up to 56 days and 60 RBC units stored in AS-1 for up to 42 days. RBCs in the two different storage solutions were processed using different types of leukoreduction filters. The RBCs in AS-1 and 60 units of RBCs in AS-7 were processed within 8 h, but the other 60 RBC units in AS-7 were processed within 2 h. Specimens were collected at three different centers.
Summary of Findings:
When blood was stored at room temperature for <2 h instead of <8 h before processing into RBCs in AS-7, lactate was higher immediately following processing (p<0.01), but no other significant effects were noted. On day 42 of storage, units in AS-7 had higher pH (p<0.01), bicarbonate (p<0.01), lactate (p<0.01), ATP (p<0.05), ATP recovery (p<0.05), and morphology index (p<0.01) and lower glucose (p<0.01) and hemolysis (p<0.01) than units stored for 42 days in AS-1. Compared to RBCs stored in AS-7 for 42 days, RBCs stored in AS-7 for 56 days had lower pH (p<0.01), glucose (p<0.05), bicarbonate (p<0.01), ATP (p<0.01), ATP recovery (p<0.01), morphology index (p<0.05), and shed microvesicle protein (p<0.001) and higher lactate (p<0.01), potassium (p<0.01), and hemolysis (p<0.01). The authors report no effect of collection center.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Protein Hematology/ auto analyzer Carbohydrate Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Cell count/volume Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 42 days
56 days
Storage Short-term storage solution AS-1
AS-7
Storage Time at room temperature <2 h
<8 h
Biospecimen Acquisition Locale of biospecimen collection 3 different centers
Biospecimen Aliquots and Components Blood processing method LEUKOSEP HWB-600-XL leukoreduction filtration
RZ2000 leukoreduction filtration