Quality of red blood cells washed using the ACP 215 cell processor: assessment of optimal pre- and postwash storage times and conditions.

Author(s): Hansen A, Yi QL, Acker JP

Publication: Transfusion, 2013, Vol. 53, Page 1772-9

PubMed ID: 23521180 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of pre- and postwash refrigerated storage and storage in AS-3 rather than SAGM on levels of supernatant potassium, ATP and hemolysis in RBCs. Blood was collected into citrate-phosphate-dextrose (CPD) tubes and held overnight before processing by the buffy coat method. RBCs were then obtained by the buffy-coat method, suspended in SAGM and washed using an ACP215 cell processor on day 2, 7, 14 or 21 and resuspended in SAGM or AS-3.

   Summary of Findings:

   Washed RBCs in SAGM or AS-3 had significantly lower Hb content than unwashed RBCs (p<0.01 and p=0.03, respectively), but there was no difference in Hb content between washed RBCs stored in SAGM and AS-3. The hematocrit and MCV were significantly higher in post-wash RBCs suspended in AS-3 than SAGM (p<0.01, both). Hemolysis was higher in RBCs washed and stored in SAGM than those stored in AS-3 (p=0.01). Hemolysis increased with pre- and postwash refrigerated storage, but the increase was greater in washed RBCs than unwashed RBCs (p<0.01). Further, the time at which the increase was significant depended on the total and individual durations of prewash and postwash refrigerated storage, with significant effects found after 28 days of combined refrigerated storage (7 days prewash and 21 days postwash or vice versa) in AS-3, after 7 days prewash and 7 days postwash in SAGM, or after 2 days prewash and 28 days postwash in SAGM. Supernatant potassium increased with refrigerated storage, but was removed by washing in SAGM or AS-3. 7 days after washing, supernatant potassium levels were higher than those found prewash on day 2. ATP levels declined with refrigerated storage of RBCs in SAGM or AS-3, but RBCs in SAGM showed slower declines in ATP levels during postwash refrigerated storage than those stored in AS-3 (p<0.01). Based on the data, a prewash refrigerated storage time of no more than 14 days was recommended.

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Spectrophotometry
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Cell count/volume	Spectrophotometry
   Protein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	2 days 5 days 7 days 14 days 21 days 28 days 35 days 42 days
   Storage	Storage conditions	Washed Unwashed
   Storage	Short-term storage solution	AS-3 SAGM
   Biospecimen Aliquots and Components	Blood processing method	Washed Unwashed

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