Preserved functional and biochemical characteristics of platelet components prepared with amotosalen and ultraviolet A for pathogen inactivation.

Author(s): Hechler B, Ohlmann P, Chafey P, Ravanat C, Eckly A, Maurer E, Mangin P, Isola H, Cazenave JP, Gachet C

Publication: Transfusion, 2013, Vol. 53, Page 1187-200

PubMed ID: 23067365 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of PCT treatment and room temperature storage on markers of Plt quality and function in PC. PC were obtained from buffy coats, placed in 35% InterSol and 65% plasma, pooled and leukoreduced. A portion of PC was PCT-treated with INTERCEPT, which uses ultraviolet A, prior to being stored at room temperature. After experimental storage, all supernatants were stored at -80 degrees C until analysis.

   Summary of Findings:

   Plt counts and expression of GPIIbIIIa, GPIaIIa and GPVI remained stable in PCT-treated and untreated PC during room temperature storage. Plt volume increased significantly during storage of PCT-treated PC (p<0.01), but it was unaffected by room temperature storage of untreated PC. pH, morphology score, GPIb alpha sites, and GPV sites declined with room temperature storage of PC, and lower levels of GPV were measured in PCT-treated PC than untreated PC on day 1.5 of room temperature storage. P-selectin, Annexin V and PAC-1 staining increased with room temperature storage and after stimulation with Thr/CVX, and PAC-1 staining was higher on day 6.5 of room temperature storage in PCT-treated PC than untreated specimens. The percentage of TMRM staining cells decreased slightly between day 1.5 and 6.5 of room temperature storage. Plt aggregation in response to ADP or collagen was stable until day 6.5, at which point it decreased in both PCT-treated and untreated PC, but Plt aggregation in response to thrombin declined in PCT-treated and untreated Plts by day 4.5. Plt aggregation in response to VWF or fibrinogen coated surfaces increased with room temperature storage of PC, regardless or PCT-treatment. Collagen-induced Plt aggregation was comparable on day 1.5 and 6.5 and between PCT-treated and untreated specimens. Storage of PC at room temperature had little effect on the intensity of two-dimensional gel spots through day 4.5, but after 6.5 days at room temperature, the intensity of 26 two-dimensional gel spots (13 identified proteins) differed by more than 1.5 fold from levels measured on day 1.5 in untreated specimens, and PCT-treated PC had changes in an additional 13 spots (4 identified proteins). By day 2.5 of room temperature storage, the intensity of 4 protein spots differed between PCT-treated and untreated PC, and no additional spots were identified after further storage.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry
   Cell count/volume	Hematology/ auto analyzer
   Glycoprotein	Flow cytometry
   Morphology	Light scattering
   Protein	1D/2D gels
   Protein	Flow cytometry
   Protein	MS/MS
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	1.5 days 2.5 days 4.5 days 6.5 days
   Storage	Storage conditions	Untreated PCT-treated
   Biospecimen Aliquots and Components	Blood processing method	Ultraviolet A treated Untreated

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