In vitro and in vivo quality of leukoreduced apheresis platelets stored in a new platelet additive solution.

Author(s): Dumont LJ, Cancelas JA, Graminske S, Friedman KD, Vassallo RR, Whitley PH, Rugg N, Dumont DF, Herschel L, Siegal AH, Szczepiorkowski ZM, Fender L, Razatos A

Publication: Transfusion, 2013, Vol. 53, Page 972-80

PubMed ID: 22882530 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage at room temperature and use of PAS-F on markers of Plt quality in apheresis-derived Plts. Plts stored in 100% plasma were collected using Trima Accel Version 5 software and Plts stored in PAS-F were collected using Trima Accel Version 6 software. Each subject donated one unit into each storage solution on the same day with at least a 90 min break between donations.

   Summary of Findings:

   pH decreased slightly during storage of aphresis-derived Plts at room temperature for up to 7 d and was significantly lower in specimens stored in PAS-F than plasma at all timepoints (p<0.01), but pH remained above 6.6 in all specimens, regardless of storage solution or duration. Two specimens stored in PAS-F had a higher pH on day 7 than day 5 which the authors attribute to glucose depletion. Regardless of storage solution, glucose decreased and lactate increased in aphresis-derived Plts during room temperature storage, but the lactate generation rate was lower and the glucose consumption rate was higher in Plts stored in PAS-F than plasma (p<0.001, both). Plts stored in PAS-F had slightly lower Plt morphology score at all time-points (p<0.01) and ESC and HSR after 5 and 7 days of storage (p<0.01, all) compared to Plts stored in 100% plasma. P-selectin increased with storage and was more than 20% higher in Plts stored in PAS-F than those stored in 100% plasma (p<0.01). Total volume, Plt concentration, MPV, and the amount of Plts per bag were comparable between Plts stored in plasma and those stored in a mixture of plasma and PAS-F.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Clinical chemistry/auto analyzer
   Cell count/volume	Hematology/ auto analyzer
   Morphology	Hematology/ auto analyzer
   Small molecule	pH
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	1 day 5 days 7 days
   Storage	Short-term storage solution	65% PAS-F and 35% plasma 100% plasma

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