In vitro and in vivo quality of leukoreduced apheresis platelets stored in a new platelet additive solution.
Author(s): Dumont LJ, Cancelas JA, Graminske S, Friedman KD, Vassallo RR, Whitley PH, Rugg N, Dumont DF, Herschel L, Siegal AH, Szczepiorkowski ZM, Fender L, Razatos A
Publication: Transfusion, 2013, Vol. 53, Page 972-80
PubMed ID: 22882530 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
The purpose of this study was to determine the effects of storage at room temperature and use of PAS-F on markers of Plt quality in apheresis-derived Plts. Plts stored in 100% plasma were collected using Trima Accel Version 5 software and Plts stored in PAS-F were collected using Trima Accel Version 6 software. Each subject donated one unit into each storage solution on the same day with at least a 90 min break between donations.
Summary of Findings:
pH decreased slightly during storage of aphresis-derived Plts at room temperature for up to 7 d and was significantly lower in specimens stored in PAS-F than plasma at all timepoints (p<0.01), but pH remained above 6.6 in all specimens, regardless of storage solution or duration. Two specimens stored in PAS-F had a higher pH on day 7 than day 5 which the authors attribute to glucose depletion. Regardless of storage solution, glucose decreased and lactate increased in aphresis-derived Plts during room temperature storage, but the lactate generation rate was lower and the glucose consumption rate was higher in Plts stored in PAS-F than plasma (p<0.001, both). Plts stored in PAS-F had slightly lower Plt morphology score at all time-points (p<0.01) and ESC and HSR after 5 and 7 days of storage (p<0.01, all) compared to Plts stored in 100% plasma. P-selectin increased with storage and was more than 20% higher in Plts stored in PAS-F than those stored in 100% plasma (p<0.01). Total volume, Plt concentration, MPV, and the amount of Plts per bag were comparable between Plts stored in plasma and those stored in a mixture of plasma and PAS-F.
- None (Fresh)
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Cell count/volume Hematology/ auto analyzer Morphology Hematology/ auto analyzer Small molecule pH Small molecule Clinical chemistry/auto analyzer
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 1 day
Storage Short-term storage solution 65% PAS-F and 35% plasma