NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

In vitro and in vivo quality of leukoreduced apheresis platelets stored in a new platelet additive solution.

Author(s): Dumont LJ, Cancelas JA, Graminske S, Friedman KD, Vassallo RR, Whitley PH, Rugg N, Dumont DF, Herschel L, Siegal AH, Szczepiorkowski ZM, Fender L, Razatos A

Publication: Transfusion, 2013, Vol. 53, Page 972-80

PubMed ID: 22882530 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of storage at room temperature and use of the platelet (Plt) additive solution Isoplate (PAS-F) on markers of Plt quality in apheresis-derived Plts.

Conclusion of Paper

Average pH and glucose decreased and lactate and P-selectin increased in apheresis-derived Plts with increasing storage duration at room temperature. Average pH, lactate generation rate, morphology score, extent of shape change (ESC), and hypotonic shock response (HSR) were significantly lower while glucose consumption rate and p-selectin levels were higher in Plts stored in PAS-F than plasma. Total volume, Plt concentration, mean Plt volume (MPV), and the amount of Plts per bag were comparable between specimens stored in plasma and those stored in a mixture of plasma and PAS-F.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of storage at room temperature and use of PAS-F on markers of Plt quality in apheresis-derived Plts. Plts stored in 100% plasma were collected using Trima Accel Version 5 software and Plts stored in PAS-F were collected using Trima Accel Version 6 software. Each subject donated one unit into each storage solution on the same day with at least a 90 min break between donations.

    Summary of Findings:

    pH decreased slightly during storage of aphresis-derived Plts at room temperature for up to 7 d and was significantly lower in specimens stored in PAS-F than plasma at all timepoints (p<0.01), but pH remained above 6.6 in all specimens, regardless of storage solution or duration. Two specimens stored in PAS-F had a higher pH on day 7 than day 5 which the authors attribute to glucose depletion. Regardless of storage solution, glucose decreased and lactate increased in aphresis-derived Plts during room temperature storage, but the lactate generation rate was lower and the glucose consumption rate was higher in Plts stored in PAS-F than plasma (p<0.001, both). Plts stored in PAS-F had slightly lower Plt morphology score at all time-points (p<0.01) and ESC and HSR after 5 and 7 days of storage (p<0.01, all) compared to Plts stored in 100% plasma. P-selectin increased with storage and was more than 20% higher in Plts stored in PAS-F than those stored in 100% plasma (p<0.01). Total volume, Plt concentration, MPV, and the amount of Plts per bag were comparable between Plts stored in plasma and those stored in a mixture of plasma and PAS-F.

    Biospecimens
    Preservative Types
    • None (Fresh)
    Diagnoses:
    • Normal
    Platform:
    AnalyteTechnology Platform
    Carbohydrate Clinical chemistry/auto analyzer
    Cell count/volume Hematology/ auto analyzer
    Morphology Hematology/ auto analyzer
    Small molecule pH
    Small molecule Clinical chemistry/auto analyzer
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Storage Time at room temperature 1 day
    5 days
    7 days
    Storage Short-term storage solution 65% PAS-F and 35% plasma
    100% plasma

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