Volume-reduced platelet concentrates: optimization of production and storage conditions.

Author(s): Van der Meer PF, Bontekoe IJ, Kruit G, Peeters G, van Toledo PJ, Tomson B, de Korte D

Publication: Transfusion, 2012, Vol. 52, Page 819-27

PubMed ID: 21981439 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of volume reduction on day 2 versus day 7 and storage container and duration on markers of Plt quality. 600 mL of buffy coat Plt concentrates in acid-citrate-dextrose-adenine (ACD-A) were volume reduced on day 2 or 7, resuspended in 15 mL, placed in the different storage containers and stored at room temperature.

   Summary of Findings:

   In general, during Plt storage the pH and morphology scores decreased with storage (p<0.01, and p<0.05, respectively), and pCO2 increased (p<0.01), regardless of which day the volume reduction was performed. The changes were significant at a later time-point when specimens were stored in BTHC-PVC rather than in syringes, and pCO2 was not significantly different between Plts volume reduced on day 7 and stored in BTHC-PVC. Plts volume reduced on day 7 had higher CD62P expression after 6 h than after 0 h of storage for all containers (p<0.01, all). The pH was significantly higher in Plts volume reduced on day 2 and stored for 0-6 h in BTHC-PCV bags than in Plts stored in syringes (p<0.001) or DEHP-PVC (p<0.001) bags, and the pH was lower in Plts volume reduced on day 7 and stored for 0-6 h in BTHC-PVC bags than DEHP-PVC bags (p<0.05) and was also lower in Plts stored for 1-6 h in BTHC-PVC bags than syringes (p<0.01). pO2 was higher and pCO2 was lower in specimens stored in BTHC-PVC bags than in DEHP-PVC bags (p<0.01, both) or syringes (p<0.01, both). When volume reduced on day 2, the percentage of Plts expressing CD62P was significantly lower in Plts stored in syringes or DEHP-PVC bags than BTHC-PVC bags (p<0.05 and p<0.001), but when the Plts were volume reduced on day 7, the differences were not consistent between storage containers and timepoints. The percentage of Plts expressing annexin A5 was not consistently affected by storage container. Initially, the morphology score was not significantly different between Plts in the different storage containers, but after 6 h, scores were higher in Plts stored in BTHC-PVC than those in DEHP-PVC (p<0.01) or syringes (p<0.01). Further, glucose consumption and lactate production were higher in Plts stored in syringes or DEHP-PVC than Plts stored in BTHC-PVC (p<0.001 or p<0.05, respectively). Swirling effect was maintained when Plts were stored in BTHC-PVC containers, but was lost when stored in DEHP-PVC bags or syringes.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Clinical chemistry/auto analyzer
   Cell count/volume	Hematology/ auto analyzer
   Gas	Clinical chemistry/auto analyzer
   Morphology	Macroscopic observation
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Type of storage container	Syringe DEHP-PVC bag BTHC-PVC bag
   Storage	Storage duration	0 h 1 h 3 h 6 h
   Storage	Time at room temperature	2 days 7 days

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2. Study Purpose

   The purpose of this study was to determine the effects of Plt collection method, Plt storage solution and storage duration on markers of Plt quality in volume-reduced Plts stored in BTHC-PVC bags. Buffy-coat-derived Plts were stored at room temperature in Composol, SSP+, or T-Sol, and apheresis Plts were stored at room temperature in plasma.

   Summary of Findings:

   Specimen pH declined when buffy-coat-derived Plts were stored in Composol, T-sol, or SSP+ but not when apheresis-derived Plts were stored in plasma. The pH in Plts stored in SSP+ and plasma remained above 6.0 even after 6 h of storage. Glucose consumption and lactate production were unaffected by Plt storage solution. The percentage of Plts expressing CD62P increased non-significantly with storage duration but was significantly higher after 6 h among Plts stored in T-sol than those stored in SSP+. The percentage of Plts expressing annexin V decreased with storage, but the decrease was only significant when Plts were stored in T-sol or plasma, and Plts in T-sol had a higher percentage of annexin V staining than those stored in Composol (p<0.05) or SSP+ (p<0.05). The morphology scores decreased with storage of Plts (p<0.01), but significant reductions were first observed after 3 h among specimens stored in Composol or T-Sol and after 6 h when specimens were stored in SSP+ or plasma.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer
   Gas	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Cell count/volume	Hematology/ auto analyzer
   Morphology	Macroscopic observation

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Apheresis Venipuncture
   Storage	Storage duration	0 h 3 h 6 h
   Storage	Short-term storage solution	Composol SSP+ T-Sol Plasma
   Biospecimen Aliquots and Components	Blood processing method	Apheresis Buffy-coat method

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