Impact of storage temperature and processing delays on cord blood quality: discrepancy between functional in vitro and in vivo assays.

Author(s): Louis I, Wagner E, Dieng MM, Morin H, Champagne MA, Haddad E

Publication: Transfusion, 2012, Vol. 52, Page 2401-5

PubMed ID: 22500587 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of a 72 h instead of an 18 h delay in processing of cord blood on cell recovery and viability. Citrate phosphate dextrose cord blood was stored without agitation. Red blood cells (RBCs) were obtained by sedimentation, and plasma was removed with a plasma extractor. RBC and plasma-depleted leukorich products were mixed together and frozen in cryopreservation bags with 10% dimethyl sulfoxide. Specimens were stored frozen for >1 month in liquid nitrogen vapor before thawing and analysis. The temperature of specimens during the initial 12 h of storage was unspecified and assumed to be room temperature.

   Summary of Findings:

   When cord blood was held at 4 degrees C for 72 h prior to processing, the percentage of CD45+ cells staining for 7-aminoactinomycin (7-AAD) was slightly lower than in specimens processed within 12 h (p<0.001), but recovery of MNC and CD34+ cells and the percentage of CD34+ cells staining for 7-AAD were unaffected. However, when cord blood was stored at room temperature for 72 h prior to processing, recovery of MNC and CD34+ cells was significantly lower than in specimens processed within 12 h (p<0.001, both) or specimens held at 4 degrees C (p<0.01 and p<0.001, respectively). Further, specimens stored for 72 h at room temperature had a lower percentage of CD45+ and CD34+ cells staining for 7-AAD than specimens stored for <12 h (p<0.01) and fewer 7-AAD stained CD34+ cells than specimens stored for 72 h at 4 degrees C (p<0.01). A 72 h delay in processing at room temperature or 4 degrees C had no effect on the ability of CD34+ cells to form colony-forming cells.

   Biospecimens

   • Cell - Cord Blood
   • Bodily Fluid - Cord Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	4 degrees C 20-24 degrees C
   Storage	Storage duration	<12 h 72 h

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