A rest period does not affect in vitro storage properties in apheresis platelets collected from the buffy coat layer.

Author(s): Wagner SJ, Seetharaman S, Kurtz J

Publication: Transfusion, 2012, Vol. 52, Page 2427-31

PubMed ID: 22452313 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of a rest period before agitation on markers of Plt quality during subsequent room temperature storage of apheresis Plts. Plts were collected in acid-citrate-dextrose (ACD) and placed on a reciprocal agitator 0, 1, 2, 4 or 6 h after collection.

   Summary of Findings:

   Resting period before storage with agitation did not affect Plt content, MPV, percentage of HSR, ESC, Plts expressing CD62P, or Plts expressing CD63, or levels of bicarbonate, glucose or lactate measured on day 1 of room temperature storage. However, PO2 was higher in Plts held for 4 or 6 h before agitation than in Plts immediately agitated (p<0.05, both) and PCO2 was higher in Plts held for 4 h than in Plts held for 0 h before agitation (p<0.05). On day 1, the percentage of discoid Plts was lower in Plts subjected to a 2 h agitation delay than in Plts agitated immediately (p<0.05), but 4 or 6 h delays before agitation had no effects on the percentage of discoid Plts. On day 5 of room temperature of storage, only glucose levels and ESC were significantly different after 4 and 6 h agitation delays, respectively, than in plts agitated immediately after collection (p<0.05, both). After 7 days of room temperature storage, PCO2 was higher in specimens with a 1 or 2 h delay before agitation than in specimens agitated immediately (p<0.05), and glucose was lower when Plts were held for 4 h prior to agitation than when agitated immediately or after a 6 h delay (p<0.005, both). Further, after 7 days of room temperature storage, the percentage of Plts expressing CD62P was higher in specimens subjected to a 2 h agitation delay than a 6 h delay (p<0.05).

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry
   Cell count/volume	Hematology/ auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Small molecule	pH
   Gas	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Morphology	Light scattering

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	1 day 5 days 7 days
   Biospecimen Aliquots and Components	Biospecimen mixing	Immediately mixed Mixing delayed by 1 h Mixing delayed by 2 h Mixing delayed by 4 h Mixing delayed by 6 h

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