The influence of maintaining the correct whole blood-to-anticoagulant ratio during donation on the quality of leukoreduced whole blood.

Author(s): Karger R, Lukow C, Kretschmer V

Publication: Transfusion, 2011, Vol. 51, Page 1486-92

PubMed ID: 21275999 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of maintaining anticoagulant to blood ratio during blood collection rather than conventional collection on markers of RBC quality. Analytes were evaluated on the collection day and during subsequent storage at 4 degrees C. Specimens were collected from 10 blood donors of each gender and blood type (O or A D+) for each anticoagulant addition method. Male donors had hemoglobin (Hb) above 135 g/L and females had Hb above 125 g/L at their last 2 blood donations. CPDA-1 was continuously added with a roller pump to maintain the CPDA-1:blood ratio or blood was collected conventionally into bags containing CPDA-1. All blood was stored at 4 degrees C and leukoreduced 2 h after collection.

   Summary of Findings:

   ATP levels, RBC aggregability, and factor VIII levels declined with increasing storage duration, but the effects of storage on other parameters were not discussed. On day 0, analytes were similar between the blood collected with continuous addition of CPDA-1 and blood collected conventionally, but trends toward more variability in Hb and lower AI were observed among specimens collected conventionally compared to specimens collected with continuous CPDA-1 addition. After storage of RBCs, levels of ATP, 2,3-diphosphoglycerate (2,3 DPG), potassium, glucose, lactate, free Hb, factor V, Factor VIII, fibrinogen, antithrombin (AT), and thrombin-AT complex, as well as activated partial thromboplastion time (APTT), prothrombin time (PT), thrombin time (TT), hemolysis rate, and pH were not significantly different in specimens collected with continuous addition of anticoagulant rather than collected conventionally. However, the aggregation amplitude was significantly higher and there was a trend of higher AI and lower time to 50% peak intensity (t1/2) in the conventionally collected specimens than the specimens collected with continuous addition of CPDA-1, but no differences in elongation index (EI) were observed. The difference in AI between collection groups was significant on day 21 (p0.03) but then decreased with additional storage up to 49 days while the difference in amplitude increased with storage duration.

   Biospecimens

   • Bodily Fluid - Blood
   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Small molecule	Enzyme assay
   Electrolyte/Metal	Flame emission photometry
   Glycoprotein	Clinical chemistry/auto analyzer
   Protein	Hematology/ auto analyzer
   Protein	Enzyme assay
   Protein	Clinical chemistry/auto analyzer
   Peptide	Hematology/ auto analyzer
   Morphology	Hematology/ auto analyzer
   Cell count/volume	Flow cytometry
   Cell count/volume	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 days 7 days 21 days 35 days 42 days 49 days
   Biospecimen Acquisition	Anticoagulant	Citrate phosphate dextrose adenine 1 Continuously added In tube/bag

   View more