Storing apheresis platelets without agitation with simulated shipping conditions during two separate periods: immediately after collection and subsequently between Day 2 and Day 3.

Author(s): Moroff G, Kurtz J, Seetharaman S, Wagner SJ

Publication: Transfusion, 2011, Vol. 51, Page 636-42

PubMed ID: 20849403 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storing apheresis Plts from two different devices without agitation immediately after collection and again between day 2 and 3 of storage on markers of Plt quality. Apheresis Plts were obtained with the Amicus and Trima separators which have blood to acid citrate dextrose (ACD) ratios of 10:1 and 11:1, respectively. During room temperature storage, Plts were agitated continuously at 65-70 cycles/min starting either within 30 min of collection or after storage at room temperature in a shipping box without agitation for 7-8 h. A subset of specimens was also subjected to a 24 h break in agitation between day 2 and 3 when they were placed back in a shipping box. PO2 and PCO2 levels were determined after <30 min, 8 h, 1 day, 2 days, 3 days, 5 days and 7 days. All other analytes were assessed only after 1, 3 and 5 days.

   Summary of Findings:

   During room temperature storage of Plts with continuous agitation pH, %disc, ESC, HSR, and PCO2 declined, and the percentage of Plts expressing CD62P increased. The PO2 declined during the first 8 h of storage, but then levels gradually increased toward those measured after <30 min. Delaying the start of agitation of Plts by 7-8 h alone had no impact on any of the markers of Plts quality. When Amicus Plts were stored at room temperature with two periods of storage without agitation, pH, %disc and ESC were lower at all time points investigated (days 1, 5 and 7) than in continuously agitated Plts (p<0.001, all). In addition, glucose was lower and pH was higher on day 5 (p<0.001), and PCO2 was higher on day 3 than in continuously agitated Plts. When Trima Plts were stored at room temperature with two periods of storage without agitation, pH and %disc were lower on days 1 and 5, ESC, HSR and glucose were lower on day 5, lactate was higher on day 5, and PCO2 was higher immediately following each period of storage without agitation than in continuously agitated Plts (p<0.001, all). The mean Plt volume (MPV), Plt concentration, PO2 and the percentage of Plts expressing CD62P were comparable at all time-points in continuously agitated Plts and those with interruptions in agitation.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Clinical chemistry/auto analyzer
   Cell count/volume	Flow cytometry
   Cell count/volume	Hematology/ auto analyzer
   Gas	Clinical chemistry/auto analyzer
   Small molecule	pH
   Small molecule	Clinical chemistry/auto analyzer
   Morphology	Light scattering
   Morphology	Light microscopy
   Morphology	Macroscopic observation

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	< 30 min 8 h 1 day 2 days 3 days 5 days 7 days
   Storage	Storage conditions	With agitation Without agitation
   Biospecimen Aliquots and Components	Blood processing method	Amicus seperator Trima seperator

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