Impact of pathogen reduction technology and storage in platelet additive solutions on platelet function.

Author(s): Galan AM, Lozano M, Molina P, Navalon F, Marschner S, Goodrich R, Escolar G

Publication: Transfusion, 2011, Vol. 51, Page 808-15

PubMed ID: 20977485 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of PRT treatment and the use of PAS on PLT function during storage of apheresis PCs at room temperature on a flatbed agitator. PRT was performed with an energy equivalent of 6.2 J/mL using a Mirasol PRT, after which PAS was added.

   Summary of Findings:

   PLT counts were unaffected by storage of PCs for 5 days, regardless of PRT treatment, but after 7 days, PLT counts were lower in PCs that were PRT treated and stored in PASIII or PASIIIM (p<0.05, both). pH decreased in PCs that were PRT treated and stored in PASIIIM or PASIII after 3 and 5 days of storage, respectively (p<0.01, both), but pH still remained within the recommended range during storage. pH was not affected by storage of untreated PCs or PCs stored in plasma. Glucose decreased and lactate increased with storage, regardless of PRT treatment and storage solution (all p<0.05), but at day 7, glucose was lower and lactate was higher in PRT treated PCs than in untreated PCs, in PCs in PASIIIM than those stored in plasma, and in PCs in PASIII than those stored in PASIIIM. Swirl effect was decreased in PRT treated PCs stored for 7 days in PASIII or PASIIIM compared to day 0 levels (p<0.01) and untreated PCs (p<0.05). GPIb was comparable in all specimens and remained constant until day 5, after which point GPIb decreased significantly in PRT treated PCs in PASIII or PASIIIM. Glycoprotein IIb-IIIa (GPIIb-IIIa) was unaffected by storage duration, PRT treatment, or storage solution. CD62P, CD63, FV/Va, and annexin V binding increased during storage of all PCs, but CD62P and CD63 plateaued before day 7 and CD62P, CD63, and annexin V binding tended to be higher in PRT treated and PAS stored PCs than other PCs. PLT reactivity was similar in PRT treated and untreated PCs and in PCs stored in PAS or plasma after 0 or 5 days, but after 7 days, adhesions were reduced in PRT treated PCs stored in PASIII compared to untreated PCs in plasma (p<0.05), and adhesions were reduced in all PCs stored in PASIII and PRT treated PCs in PASIIIM compared to day 0 (p<0.05, all).

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer
   Cell count/volume	Flow cytometry
   Protein	Flow cytometry
   Carbohydrate	Clinical chemistry/auto analyzer
   Morphology	Macroscopic observation
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 days 3 days 5 days 7 days
   Storage	Storage conditions	PRT treated Untreated
   Storage	Short-term storage solution	Plasma PASIII PASIIIM
   Biospecimen Aliquots and Components	Blood processing method	PRT treated Untreated

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