Identification of lipids that accumulate during the routine storage of prestorage leukoreduced red blood cells and cause acute lung injury.

Author(s): Silliman CC, Moore EE, Kelher MR, Khan SY, Gellar L, Elzi DJ

Publication: Transfusion, 2011, Vol. 51, Page 2549-54

PubMed ID: 21615744 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage and leukoreduction of RBCs on the lipid profile of the supernatant and the PMN-priming activity of the extracted lipids. Specimens were leukoreduced by filtration. After experimental storage, supernatants were kept at -80 degrees C until analysis.

   Summary of Findings:

   PMN-priming activity of the RBC supernatant lipids, as determined by superoxide anion production, increased with increasing storage of LR and NLR-RBCs. Both LR and NLR-RBCs had increased PMN-priming activity after only 14 days of storage (p<0.05), but in LR-RBCs, the priming activity only increased slightly between days 28 and 42, while it increased continuously until day 42 in NLR-RBCs. While NLR-RBCs contained two lipid peaks (nonpolar lipids and lyso PCs), LR-RBCs only had a peak corresponding to nonpolar lipids. Arachidoic acid, 5-HETE, 12-HETE and 15 HETE increased during storage (p<0.05), but the increased 15-HETE did not effect PMN-priming activity.

   Biospecimens

   • Bodily Fluid - Blood
   • Cell - Red Blood Cells

   Preservative Types

   • Frozen
   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Lipid	HPLC-MS
   Gas	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 days 14 days 21 days 28 days 35 days 42 days
   Biospecimen Aliquots and Components	Blood and blood products	Leukoreduced red blood cells Non-leukoreduced red blood cells
   Biospecimen Aliquots and Components	Blood processing method	Leukoreduced Non-leukoreduced

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