Effect of additive solutions on red blood cell (RBC) membrane properties of stored RBCs prepared from whole blood held for 24 hours at room temperature.

Author(s): Veale MF, Healey G, Sparrow RL

Publication: Transfusion, 2011, Vol. 51 Suppl 1, Page 25S-33S

PubMed ID: 21223292 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage solution and duration on the quality of RBCs produced after storage of blood at room temperature for 24 h. CPD was rapidly cooled to 20 degrees C and held for 24 h prior to leukoreduction and isolation of the RBCs. Packed RBCs were suspended and stored at 4 degrees C in 100 mL SAG-M, 110 mL PAGGSM or 110 mL Erythrosol-4.

   Summary of Findings:

   During refrigerated storage of RBCs for up to 49 days, the pH and FSC declined, and hemolysis, supernatant potassium, GPA+ MPs, and adhesion to ECs increased, but SSC did not change. MCV increased with refrigeration of RBCs in SAG-M, decreased with refrigeration in Erythrosol, and remained stable during refrigeration of RBCs in PAGGSM. Compared to RBCs in SAG-M, RBCs in Erythrosol-4 or PAGGSM had lower pH, hemolysis, FSC, MCV, GPA+ MP counts, osmotic fragility, and adhesion to ECs and higher SSC at at least one time-point during the 49 days of refrigerated storage. At one or more time-points during refrigerated storage, RBCs in Erythrosol-4 had higher supernatant potassium levels and lower Annexin V binding than RBCs in SAG-M or PAGGSM.

   Biospecimens

   • Bodily Fluid - Blood
   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry
   Cell count/volume	Hematology/ auto analyzer
   Protein	Spectrophotometry
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Morphology	Flow cytometry
   Morphology	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	1 day 7 days 14 days 28 days 42 days 49 days
   Storage	Prefixation freezing	SAG-M PAGGSM Erythrosol-4

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