Characterization of blood components separated from donated whole blood after an overnight holding at room temperature with the buffy coat method.

Author(s): Lu FQ, Kang W, Peng Y, Wang WM

Publication: Transfusion, 2011, Vol. 51, Page 2199-207

PubMed ID: 21492181 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of an overnight delay in processing whole blood using the buffy coat method and storage of blood components on markers of quality in RBCs, PCs, and plasma. Blood was collected in citrate-phosphate-dextrose-adenosine (CPDA) and stored at room temperature before separation into components using a two-step centrifugation method. After separation, RBCs were stored at 4 degrees C for 42 days and analyzed every 7 days, the plasma was frozen at -80 degrees C for an unspecified time, and the PCs were stored at 22 degrees C for 5 days with daily analysis.

   Summary of Findings:

   Initial RBC count, hematocrit (Hct), residual WBC count and hemoglobin (Hb) content were unaffected by keeping whole blood specimens overnight at room temperature before processing. During storage of RBCs at 4 degrees C for 42 days, the rate of hemolysis, potassium levels and lactate levels increased, and pH, DPG and ATP levels decreased. Specimens held overnight before processing had lower 2,3 DPG levels on days 1-21, lower pH on days 1-7, lower supernatant potassium levels on days 14-42, higher ATP levels on days 1-7 and higher lactate levels on days 1-14 (p<0.05, all). PC yield was lower from whole blood specimens stored for 6-8 h at room temperature than from specimens held overnight at room temperature, but PC volume and residual WBC count were not affected by an overnight delay instead of a 6-8 h delay before processing. During storage at 22 degrees C for 5 days, PCs showed declines in the extent of shape change, pO2, pCO2 and glucose levels and increases in annexin V binding, CD62P expression and lactate levels. When whole blood was held overnight before processing and subsequent storage of PC at 22 degrees C, the lactate levels were higher on days 1-2, HSR was higher on days 4-5, and PLT activation, assessed by annexin V binding, and CD62P expression were lower on days 1-2 than in PC from whole blood processed within 8 h. Of the 9 coagulation factors investigated, only factor VIII levels in plasma were reduced when whole blood was stored overnight at room temperature before analysis instead of processed within 6-8 h of collection, but even after the decline, factor VIII levels met national standards for clinical use.

   Biospecimens

   • Bodily Fluid - Blood
   • Cell - Red Blood Cells
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Gas	Clinical chemistry/auto analyzer
   Small molecule	Bioassay
   Small molecule	HPLC
   Small molecule	Clinical chemistry/auto analyzer
   Small molecule	Spectrophotometry
   Carbohydrate	Clinical chemistry/auto analyzer
   Cell count/volume	Flow cytometry
   Cell count/volume	Hematology/ auto analyzer
   Cell count/volume	Spectrophotometry
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Glycoprotein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 days 1 days 2 days 3 days 4 days 5 days 7 days 14 days 21 days 28 days 35 days 42 days
   Storage	Time at room temperature	6-8 h Overnight
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Platelets Red blood cells
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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