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Comprehensive proteomic analysis of protein changes during platelet storage requires complementary proteomic approaches.

Author(s): Thon JN, Schubert P, Duguay M, Serrano K, Lin S, Kast J, Devine DV

Publication: Transfusion, 2008, Vol. 48, Page 425-35

PubMed ID: 18067510 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage and analysis method on the proteomic profile of platelet concentrates. The temperature at which the platelet concentrates were stored was not provided.

   Summary of Findings:

   In total, 503 proteins were identified to be differentially affected by storage for 7 days versus 1 day using the three methods. 355 proteins were identified by isobaric tags for relative and absolute quantification (iTRAQ) labeling, 139 were identified by isotope-coded affinity tagging (ICAT) and 93 were identified by 2-dimensional gel electrophoresis/differential gel electrophoresis (2D gel/DIGE). Only 5 proteins were common to all three methods, and only one of these showed the same direction of change in all three methods. Of the 27 proteins identified to be affected by storage using both ITRAQ and ICAT, the direction of change was the same for 22 of the proteins, but only 8 of the 44 proteins identified by both 2D gel/DIGE and ITRAQ to be differentially expressed between storage times showed changes in the same direction for both analysis methods. None of the 3 proteins identified by both 2D gel/DIGE and ICAT to be affected by storage duration showed changes in the same direction for both analysis methods. Western blot confirmed increases in Superoxide dismutase, Rho-GDP dissociation inhibitor, Septin Z, and Zyxin in platelet concentrates stored for 7 days rather than 1.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	1D/2D gels
   Protein	LC-ESI-MS/MS
   Protein	Western blot

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	1 day 7 days
   1D/2D gels Specific	Technology platform	MS with ITRAQ labeling MS with ICAT labeling
   Western blot Specific	Targeted peptide/protein	Superoxide dismutase Rho-GDP dissociation inhibitor Septin Z Zyxin

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