NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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The quality of fresh-frozen plasma produced from whole blood stored at 4 degrees C overnight.

Author(s): Cardigan R, Lawrie AS, Mackie IJ, Williamson LM

Publication: Transfusion, 2005, Vol. 45, Page 1342-8

PubMed ID: 16078924 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of storing whole blood overnight on coagulation variables in fresh frozen plasma (FFP).

Conclusion of Paper

Storage of whole blood at 4 degrees C for 18-24 hours prior to separation of plasma led to some significant changes in coagulation variables, however, in general changes were clinically insignificant and greater than 95% of specimens gave measurements that fell within a reference range determined by specimens processed within 8 hours of blood collection. The use of LST2 whole blood filters for leukodepletion (LD) resulted in 20% higher prothrombin fragment 1 + 2 (Pro F1 + 2) levels and 10% lower factor (F)XI levels. The authors believe that storage of whole blood overnight before the preparation of FFP is acceptable for most coagulation analysis.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of storing whole blood at 4 degrees C for 18-24 hours prior to LD, centrifugation, and separation of plasma on coagulation variables in FFP. In addition different whole blood filters used for LD were examined.

    Summary of Findings:

    Storage of whole blood at 4 degrees C for 18-24 hours prior to separation of plasma led to significant decreases in fibrinogen (12%), FV (15%), FVIII (23%), and FXI (7%) and significant increases in the activated partial thromboplastin time (APTT) ratio and levels of Pro F1 + 2 when compared to historical data of specimens processed and frozen within 8 hours of collection. Clinically insignificant reductions were also seen after storage of whole blood for 18-24 hours in levels of antithrombin III (ATIII), protein S (PS), and alpha2-antiplasmin (alpha2-AP). However, more than 95% of specimens stored for 18-24 hours had levels of FII, FV, FVII, FVIII, FIX, FX, FXI, FXII, ATIII, PS, and alpha2-AP within the reference range based on specimens processed within 8 hours. Interestingly, 9 out of 14 specimens with Pro 1 + 2 levels greater than 1.10 nmol/L were processed using the LST2 filter and FXI levels were 10% lower with these filters compared to RZ2000, or NPBI T2926 whole blood filters.

    Biospecimens
    Preservative Types
    • Frozen
    Diagnoses:
    • Not specified
    • Normal
    Platform:
    AnalyteTechnology Platform
    Protein Hematology/ auto analyzer
    Morphology Hematology/ auto analyzer
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Acquisition Anticoagulant Citrate-phosphate-dextrose
    Sodium citrate
    Preaquisition Patient genotype Blood type O
    Blood type A
    Storage Storage duration 18 to 24 h
    8 h
    Storage Storage temperature 4 degrees C
    Biospecimen Aliquots and Components Filtration RZ2000
    LST2
    NPBI T2926
    Pall LPSI
    Baxter plasma filters
    WBSP
    LST1
    Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated
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