Vitamin K-dependent coagulation factors and fibrinogen levels in FFP remain stable upon repeated freezing and thawing.
Author(s): Ben-Tal O, Zwang E, Eichel R, Badalbev T, Hareuveni M
Publication: Transfusion, 2003, Vol. 43, Page 873-7
PubMed ID: 12823746 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of storage, freeze-thaw cycling, and blood group on coagulation factors in plasma.
Conclusion of Paper
Storage and freeze-thaw cycling had no effects on average prothrombin, factor VII (FVII), factor IX (FIX), factor X (FX) and fibrinogen levels. Generally, there were no significant effects of blood group, but prothrombin was above the normal range in group B plasma, and FVII was significantly different between blood groups after 1 freeze-thaw cycle and after 24 h of storage at 4 degrees C.
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage, freeze-thaw cycling, and blood group on coagulation factors in plasma. Blood was stored for 18-24 h at 18 degrees C, after which the separated plasma was first stored at -35 degrees C for 2 months and then stored at -80 degrees C for up to 6 months, prior to this study.
Summary of Findings:
When the plasma was first thawed, 19 of 20 specimens had prothrombin, FVII, FIX, FX and fibrinogen levels within the normal range. The remaining specimen showed 61.3% of normal FVII levels (normal range 63-139%) after first thaw. The average levels of prothrombin, FVII, FIX, FX and fibrinogen remained within normal ranges after storage for 24 h at 4 degrees C, followed by a week at -80 degrees C, thawing, and a second storage for 24 h at 4 degrees C. While there were slight differences in average levels of FII, FVII, and FX between the blood groups, all levels remained within normal ranges at each timepoint. Prothrombin was above the normal range in group B plasma at each timepoint, and FVII was significantly different between blood groups after 1 freeze-thaw cycle and after 24 h of storage at 4 degrees C.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Glycoprotein Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Morphology Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Patient genotype Type A blood
Type B blood
Type AB blood
Type O blood
Storage Storage temperature 4 degrees C
-80 degrees C
Storage Storage duration 0 h
24 h
1 week
Storage Freeze/thaw cycling 1 cycle
2 cycles