Evaluation of polyethylene terephthalate for ABO and Rh typing and alloantibody screening.

Author(s): Anderson DR, Wiseman J, MacLeod J, Burton E, Zayed E

Publication: Transfusion, 2000, Vol. 40, Page 669-72

PubMed ID: 10864986 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collection tube type, anticoagulant, refrigerated storage and testing platform on ABO and Rh typing and alloantibody screening of blood specimens.

   Summary of Findings:

   Collection tube type, anticoagulant (K2EDTA), and storage for up to 28 days had no effects on ABO and Rh typing. However, the strength of the reaction to B cells and anti-D were affected by storage when the tube system, but not the gel card system, was used. In the two specimens with alloantibodies, the alloantibodies were found regardless of collection tube or storage duration.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Light microscopy
   Protein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Silica coated plastic Plastic Glass
   Storage	Storage temperature	2-8 degrees C
   Storage	Storage duration	< 3 h 1 day 2 days 21 days 28 days
   Immunoassay Specific	Technology platform	Gel card Tube system
   Biospecimen Acquisition	Anticoagulant	None Potassium EDTA

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