The viability of autologous human red cells stored in additive solution 5 and exposed to 25 degrees C for 24 hours.
Author(s): Reid TJ, Babcock JG, Derse-Anthony CP, Hill HR, Lippert LE, Hess JR
Publication: Transfusion, 1999, Vol. 39, Page 991-7
PubMed ID: 10533826 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of refrigerated storage and exposure to room temperature for 24 h on day 13 or 27 on the quality of RBCs stored in AS-5. Specimens were assayed before and after exposure to room temperature for 24 h on day 13-14 and 27-28. Deproteinized supernatants were stored at -80 degrees C until assay for glucose and ATP.
Summary of Findings:
During refrigerated storage, glucose decreased by 7.4 mg/dL per day, and warming RBCs to room temperature for a 24 hour period increased the rate of decrease to 86 mg/dL a day. pH declined by 0.7 during the 45 days of refrigerated storage, with a slightly larger decrease noted in specimens that were warmed to room temperature for 24 h on day 13 or 28. The ATP concentration remained relatively stable during refrigerated storage until day 20 or 25 but declined more rapidly when RBCs were exposed to room temperature for 24 h. Hemoglobin increased with refrigerated storage, regardless of room temperature exposure. None of the specimens in this study showed bacterial contamination.
Biospecimens
Preservative Types
- Other Preservative
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Cell count/volume Hematology/ auto analyzer Cell count/volume Microbiological assay Protein Hematology/ auto analyzer Protein Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 days
13 days
14 days
21 days
27 days
28 days
5 weeks
6 weeks
Storage Time at room temperature 0 h
24 h on day 13-14
24 h on day 27-28