Manufacture of red cells in additive solution from whole blood refrigerated for 5 days or remanufactured from red cells stored in plasma.
Author(s): Wiltshire M, Cardigan R, Thomas S
Publication: Transfus Med, 2010, Vol. 20, Page 383-91
PubMed ID: 20681977 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of refrigerated storage of RCC in CPD for 5 or 6 days prior to refrigerated storage in SAGM and subsequent irradiation of RCC on day 14 on markers of RCC quality. Values were compared to published reference values for RCC stored in SAGM from day 0. The storage duration is the total storage duration such that value for days 1-5 or 6 are for RCC in CPD.
Summary of Findings:
RCC stored in CPD plasma for 5 or 6 days before replacement of the storage solution with SAGM had comparable hematocrit but slightly lower volume and hemoglobin content than published reference data based on storage of RCC in SAGM from day 0 on. Hemolysis rates increased with refrigerated storage of RCC transferred to SAGM on day 5 or 6, but rates were only significantly higher than reference rates in specimens transferred to SAGM on day 5 and stored for 35 or 42 days (p<0.05, and p<0.001, respectively). Supernatant potassium levels also increased with refrigerated storage, but after 35 and 42 days, potassium levels were lower in specimens stored in CPD until day 6 and then placed into SAGM than reference specimens stored in SAGM the entire time (p<0.001, both). ATP and 2,3-DPG levels decreased during refrigerated storage of RCC, regardless of whether or not they were stored in CPD prior to storage in SAGM. The volume and hemoglobin content of specimens transferred to SAGM on day 5 were unaffected by irradiation on day 14, but the hematocrit was lower in irradiated RCC than non-irradiated RCC (p<0.05). Hemolysis increased during storage of irradiated specimens, and by day 28 (14 days post-irradiation), all irradiated RCC had hemolysis levels >0.8% compared to only 15% of non-irradiated specimens transferred to SAGM on day 5 or 6 having hemolysis levels >0.8%.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Cell count/volume Spectrophotometry Electrolyte/Metal Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Small molecule Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Short-term storage solution CPD
SAGM
Storage Storage duration 1 day
2 days
5 days
6 days
7 days
14 days
28 days
35 days
42 days
Biospecimen Aliquots and Components Blood processing method Not irradiated
Irradiated
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Study Purpose
The purpose of this study was to determine the effects of refrigerated storage of leukodepleted whole blood for 5 days prior production of RCC in SAGM and on markers of RCC quality. Values were compared to published reference values for RCC stored in SAGM from day 0.
Summary of Findings:
When RCC were produced in SAGM after refrigeration of leukodepleted whole blood for 5 days at 4 degrees C, the volume, hematocrit and hemoglobin content were comparable to published reference values based on RCC stored in SAGM from day 0 (no prior storage of leukodepleted whole blood). After 42 days of storage in SAGM, RCC from leukoreduced blood, previously stored for 5 days at 4 degrees C, had similar hemolysis rates, 2,3-DPG levels and similar or lower supernatant potassium levels compared to published reference values. ATP levels in RCC from leukoreduced blood that was previously stored for 5 days at 4 degrees C were initially lower than published reference values, but by day 42 of storage, levels were comparable.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Cell count/volume Spectrophotometry Electrolyte/Metal Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Small molecule Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 1 day
2 days
5 days
6 days
7 days
14 days
28 days
35 days
42 days
Biospecimen Aliquots and Components Blood and blood products Leukocyte reduced blood
Red blood cells
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Study Purpose
The purpose of this study was to determine the effects of production of RCC in CPD versus CPDA-1 and refrigerated storage duration on markers of RCC quality. RCC in CPD were stored for up to 28 days, while RCC in CPDA-1 were stored for up to 35 days.
Summary of Findings:
RCC from whole blood collected into CPD or CPDA-1 met the specifications for volume and the original guideline of a hematocrit between 0.50 and 0.60, but only 25% of specimens met the more stringent requirement of a hematocrit between 0.50-0.55. Hemolysis rates met guidelines and were comparable between specimens collected in CPD and CPDA-1. Supernatant potassium levels increased, and ATP levels decreased with refrigerated storage of RCC in CPD and CPDA-1, but the rate of change was significantly greater when units were stored in CPD than CPDA-1 (p<0.05, both). 2,3-DPG levels were lower in RCC stored in CPDA-1 than CPD (p<0.01).
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Cell count/volume Spectrophotometry Electrolyte/Metal Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Small molecule Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 1 day
2 days
6 days
7 days
14 days
28 days
35 days
Biospecimen Acquisition Anticoagulant Citrate phosphate dextrose adenine 1
Citrate-phosphate-dextrose