Protein stability of previously frozen plasma, riboflavin and UV light-treated, refrozen and stored for up to 2 years at -30 °C.

Author(s): Ettinger A, Miklauz MM, Hendrix BK, Bihm DJ, Maldonado-Codina G, Goodrich RP

Publication: Transfus Apher Sci, 2011, Vol. 44, Page 25-31

PubMed ID: 21251884 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of frozen storage duration before and after pathogen inactivation with riboflavin and UV light on 15 protein factors, anticoagulant proteins, and inhibitor proteins in plasma. Apheresis acid-citrate-dextrose plasma and citrate-phosphate-dextrose plasma obtained from whole blood was held at 4 degrees C, for up to 8 h, before being flash-freezing at -30 degrees C. After the first storage period, plasma was thawed in a plasma defroster for up to 2 h, and an aliquot was removed (control) before the remaining plasma was treated with riboflavin and UV light exposure. Both the treated plasma and control plasma were then flash-frozen and stored at -30 degrees C for the second storage period.

   Summary of Findings:

   Protein levels in apheresis plasma and plasma collected from whole blood were not significantly different. Generally, 70-100% of the analyte levels in the storage duration-matched, untreated, control specimens were retained in the riboflavin and UV light-treated specimens, but levels of fibrinogen, factor VIIc, factor XI, and factor VII were retained at lower levels in the riboflavin and UV light-treated plasma (68-90%, 66-87%, 52-86%, 65-77%, respectively) compared to untreated control specimens with the same storage durations. After storage for 2 years (either 3 months pretreatment and 21 months post-treatment or 21 months pretreatment and 3 months post-treatment), compared to control specimens, riboflavin and UV light-treated plasma had similar total protein levels, decreased factor XI (53%) and factor VII levels (65%), and 70-100% of the levels measured in control specimens for all other proteins. Anticoagulant and inhibitor proteins were particularly stable in riboflavin and UV light-treated plasma, and 85-100% of the levels found in the untreated specimens were retained with the same storage.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer
   Protein	Spectrophotometry
   Glycoprotein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Venipuncture Apheresis
   Storage	Storage duration	3 weeks and 3 weeks 4 weeks and 9 weeks 9 weeks and 4 weeks 2 months and 4 months 3 months and 9 months 9 months and 3 months 3 months and 21 months 21 month and 3 months
   Biospecimen Acquisition	Pre-preservation condition	Riboflavin and UV light exposure No riboflavin and UV light exposure
   Biospecimen Aliquots and Components	Blood processing method	Riboflavin and UV light exposure No riboflavin and UV light exposure

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