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Investigating the effect of a platelet additive solution on apheresis platelet and fibrin network ultrastructure.

Author(s): Pretorius E, Crookes R, Oberholzer HM, van der Spuy WJ

Publication: Transfus Apher Sci, 2010, Vol. 43, Page 347-51

PubMed ID: 21036668 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of storage of platelets (plts) in citrated plasma (CP) or 60% CP and 40% platelet additive solution (CP/PAS) at 22 degrees C or storing plts in CP/PAS without temperature control or agitation on plt quality, morphology and aggregation.

Conclusion of Paper

After 1, 3, or 5 days of storage at 22 degrees C with agitation, plts in CP or CP/PAS had normal morphology, pH, and clot structure, but during storage at 22 degrees C, annexin V and CD62P expression increased. In contrast, after 3 days of storage, only 2 of 12 plt specimens stored in CP/PAS without controlled temperature or agitation showed swirling effect. Further, the pH of CP/PAS plts decreased with increasing storage duration without temperature control or agitation. On day 1 of storage without temperature control, plts in CP/PAS had clots that were comparable to CP specimens, but the authors report that fibrin in CP/PAS specimens was more matted in appearance. After 3 days of storage without temperature control, plts in CP/PAS showed signs of apoptosis, and by day 5, there were no plts in these specimens.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of storage of plts in CP or CP/PAS at 22 degrees C or storing plts in CP/PAS without temperature control or agitation on plt quality, morphology and aggregation. PAS was added to plts immediately following apheresis. All plts were gamma-irradiated on day 1. Thrombin was added to induce coagulation on days 1, 3, or 5. The method by which CD62P expression was assessed was not specified.

    Summary of Findings:

    After 1, 3, or 5 days of storage at 22 degrees C with agitation, plts in CP or CP/PAS had normal morphology, pH, and clot structure, but during storage at 22 degrees C, annexin V and CD62P expression increased. In contrast, after 3 days, only 2 of 12 plt specimens stored in CP/PAS without controlled temperature or agitation displayed swirling effect. Further, the pH of CP/PAS plts decreased with increasing storage duration without temperature control or agitation. On day 1 of storage without temperature control, plts in CP/PAS had clots that were comparable to CP specimens, but the authors report that fibrin in CP/PAS specimens was more matted in appearance. After 3 days of storage without temperature control, plts in CP/PAS showed signs of apoptosis, and by day 5, there were no plts in these specimens.

    Biospecimens
    Preservative Types
    • None (Fresh)
    Diagnoses:
    • Normal
    Platform:
    AnalyteTechnology Platform
    Morphology Macroscopic observation
    Small molecule Clinical chemistry/auto analyzer
    Protein Clinical chemistry/auto analyzer
    Cell count/volume Electron microscopy
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Storage Storage duration 1 day
    3 days
    5 days
    Storage Short-term storage solution 100% citrated plasma
    40% PAS, 60% citrated plasma
    Storage Storage temperature 22 degrees C
    Uncontrolled temperature
    View more

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