Shear stress activation of platelets with subsequent refractoriness.

Author(s): Aursnes I, Sundal J, Nome T

Publication: Thromb Res, 1987, Vol. 45, Page 29-37

PubMed ID: 3563976 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of anticoagulant, time at room temperature, mixing speed, and centrifugation on platelet aggregation. Blood was obtained with minimal stasis, and the first 2 mL were discarded. Specimens were mixed with a magnetic stir bar.

   Summary of Findings:

   Platelet aggregation occurred in response to stirring without ADP addition, but the response was dependent on time at room temperature prior to mixing, with the greatest response observed in specimens held at room temperature for 17 min and the least in specimens left at room temperature for 26 min. The authors report that platelet aggregation in response to specimen mixing did not occur if the specimen was anticoagulated with citrate instead of soybean trypsin inhibitor (SBTI) or if the specimen was heated to 37 degrees C prior to mixing. The subsequent addition of ADP to specimens, after mixing-induced aggregation, resulted in only a very small additional response. When ADP was added at the commencement of stirring, the aggregation response increased with increasing stirring speed, and while the trend was the same in citrated specimens, the magnitude of the changes was smaller. The platelet concentration generally decreased at a lower sampling level within the tubes after a 3 min centrifugation, but the effect was attenuated when platelet aggregation was inhibited by EDTA, and the sampling level effect was exaggerated when SBTI-anticoagulated specimens were stimulated by the addition of ADP. Beta-thromboglobulin was released when specimens were centrifuged for 10 min.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Biospecimen mixing	0 rpm 600 rpm 1200 rpm
   Storage	Time at room temperature	8 min 17 min 26 min
   Biospecimen Acquisition	Anticoagulant	Citrate EDTA Soybean trypsin inhibitor
   Biospecimen Aliquots and Components	Biospecimen components	20 mm below top of tube 40 mm below to of tube 60 mm below top of tube
   Biospecimen Aliquots and Components	Blood and blood products	Multiple sampling positions examined

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