Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Micro-array profiling exhibits remarkable intra-individual stability of human platelet micro-RNA.

Author(s): Stratz C, Nührenberg TG, Binder H, Valina CM, Trenk D, Hochholzer W, Neumann FJ, Fiebich BL

Publication: Thromb Haemost, 2012, Vol. 107, Page 634-41

PubMed ID: 22371016 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the variability in miRNA expression in platelets over the course of 10 days, and to determine the effects of donor consumption of ASA on miRNA profiles. Platelets were prepared by leukocyte depletion of platelet rich plasma after blood was collected into trisodium citrate tubes. Platelet pellets were stored at -80 degrees C until RNA extraction using the miRNeasy Mini Kit, and eluted RNA was stored at -80 degrees C until analysis. Microarray analysis was performed using the Geniom Biochip miRNA homosapiens.

   Summary of Findings:

   All 221 miRNAs found to be expressed in platelets showed relatively stable expression over the 10 days of specimen collection, and none were significantly altered 24 hours after patients received a 500 mg dose of ASA on day 9.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   RNA	RT-PCR
   RNA	Automated electrophoresis/Bioanalyzer
   RNA	DNA microarray
   Cell count/volume	Hematology/ auto analyzer
   Cell count/volume	Flow cytometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Other drugs	500 mg ASA No ASA
   Biospecimen Acquisition	Time of biospecimen collection	Day 0 Day 1 Day 3 Day 9 Day 10

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