Overestimation of plasma level of factor V coagulant activity due to unrecognised preanalytical coagulation.

Author(s): Eschwege V, Trillard M, Robert A

Publication: Thromb Haemost, 2004, Vol. 91, Page 827-8

PubMed ID: 15045150 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of coagulation on PT, APTT, fibrinogen, factor II, factor VII + X, and factor V levels in plasma. All specimens were collected in Vacutainers containing citrate. In an effort to evaluate the effects of initially undetected preanalytical clots, specimens with obvious preanalytical clots after centrifugation were not included in the study. Instead, after centrifugation, plasma from specimens with no obvious clots was transferred into new tubes, and the pellets were poured through a gauze swab. Specimens that had a visible clot at this point were included in the study as the clotted specimen (8/1334 or 0.6%), and a second specimen (non clotted) was drawn within 1 day of the first for comparison.

   Summary of Findings:

   Higher factor V levels were measured in the plasma from clotted specimens than non clotted specimens. For the 8 specimen pais included in the study, the increases in factor V levels ranged from 46-335%. PT, APTT, fibrinogen, factor II, and factor VII + X measurements were not affected by initially undetected preanalytical clots.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	Macroscopic observation
   Morphology	Hematology/ auto analyzer
   Glycoprotein	Hematology/ auto analyzer
   Protein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Clotted specimen Non clotted specimen

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