The effects of quality and timing of venepuncture on markers of blood coagulation in healthy middle-aged men.

Author(s): Miller GJ, Bauer KA, Barzegar S, Foley AJ, Mitchell JP, Cooper JA, Rosenberg RD

Publication: Thromb Haemost, 1995, Vol. 73, Page 82-6

PubMed ID: 7740501 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of time of day of blood collection and difficulty of blood draw on factor VII activity, fibrinogen, F1+2, and FPA measurements in plasma. The difficulty of blood draw was graded either satisfactory, not fully satisfactory, or unsatisfactory. Characteristics of an unsatisfactory blood draw included drawing less than 22 mL of blood as well as one or more of the following: blood flow not maintained with ease, interrupted blood flow, procedure taking longer than 2 min, or the arm of the patient was unsteady during collection. Both citrate and EDTA collection tubes were used.

   Summary of Findings:

   A not fully satisfactory blood draw or an unsatisfactory blood draw had no effects on factor VII activity or fibrinogen concentration compared to levels measured in specimens obtained with a satisfactory blood draw. However, significantly lower F1+2 and FPA levels were measured when specimens were obtained with a satisfactory blood draw than unsatisfactory or not fully satisfactory blood draws, and redrawing unsatisfactory or not fully satisfactory specimens 1 year later resulted in significant increases in levels of both analytes. Time of day of blood draw had no significant effects on fibrinogen concentration or factor VII activity in plasma. However, F1+2 levels were slightly but significantly higher when specimens were drawn between 9:00 am and 2:59 pm than they were in specimens drawn between 6:00 am and 8:59 am or 3:00 pm or later (p<0.0001). FBA levels significantly declined throughout the day (p<0.02).

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Hematology/ auto analyzer
   Glycoprotein	Hematology/ auto analyzer
   Peptide	Radioimmunoassay
   Protein	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Difficult blood draw Not a difficult blood draw
   Biospecimen Acquisition	Time of biospecimen collection	6:00-8:59 am 9:00 am-2:59 pm 3:00 pm or later

   View more