Time-dependent absorption of therapeutic drugs by the gel of the Greiner Vacuette blood collection tube.

Author(s): Dasgupta A, Yared MA, Wells A

Publication: Ther Drug Monit, 2000, Vol. 22, Page 427-31

PubMed ID: 10942183 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storing serum in Greiner SST on the measurement of therapeutic drugs. Specimens were stored at room temperature for the first 4 h and then at 4 degrees C for the next 20 h. Both patient serum specimens and spiked drug-free serum were analyzed. Serum was transferred to experimental storage tubes after clotting and centrifugation.

   Summary of Findings:

   Concentrations of tricyclic antidepressants declined by 26% when serum was stored for 24 h in Greiner SST compared with 4% when stored in plain tubes. Further studies showed that concentrations of each of the 4 tricyclic antidepressants decreased with storage in Greiner SST and that the effect was more significant on amitriptyline and imipramine than their metabolites. There was also a small decrease in carbamazepine with storage in Greiner SST, but not plain tubes. Amikacin, gentamycin, tobramycin, vancomycin, digoxin, quinidine, theophylline, phenobarbital, phenytoin, valproic acid, salicylate, acetaminophen, and ethanol concentrations were unaffected by storage in plain tubes or Greiner SST.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 h 4 h 24 h
   Storage	Storage temperature	Room temperature 4 degrees C
   Storage	Type of storage container	Greiner Vacuette with serum separator gel Plain tube

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