Pre-analytical issues for testosterone and estradiol assays.
Author(s): Raff H, Sluss PM
Publication: Steroids, 2008, Vol. 73, Page 1297-304
PubMed ID: 18589466 PubMed Review Paper? Yes
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of storing clotted blood at 2-8 degrees C prior to centrifugation on the measurement of estradiol in serum.
Summary of Findings:
Storing clotted blood at 2-8 degrees C for up to 8 days prior to centrifugation had no effect on estradiol levels in serum.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Steroid Immunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 1 day
2 days
3 days
4 days
5 days
8 days
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated
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Study Purpose
The purpose of this paper was to determine the effects of menstrual cycle day during specimen collection on the measurement of estradiol, progesterone, testosterone, LH, FSH and SHBG in serum. Details of the methods used to analyze the specimens were not included. Serum was stored frozen until analysis.
Summary of Findings:
As expected, FSH, LH and estradiol peaked in specimens collected on cycle day 0, and progesterone peaked in specimens collected around cycle day 10. Small variations in testosterone and SHBG levels were observed in serum collected on different cycle days without any peaks.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Steroid Clinical chemistry/auto analyzer Glycoprotein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Time of biospecimen collection Day 0-28 of menstrual cycle