Stability of genomic DNA in dried blood spots stored on filter paper.
Author(s): Chaisomchit S, Wichajarn R, Janejai N, Chareonsiriwatana W
Publication: Southeast Asian J Trop Med Public Health, 2005, Vol. 36, Page 270-3
PubMed ID: 15906682 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to assess the stability of DNA isolated from dried neonatal blood stored on filter paper in ambient, tropical conditions (average temperature 28 degrees C, 73% average relative humidity), and its suitability for PCR analysis. Beta-globin and PAX8 gene fragments were analyzed.
Summary of Findings:
PCR amplification of DNA extracted from dried blood spots that were stored at ambient tropical conditions for up to 11 years was successful for the majority of the specimens analyzed. PCR amplification of 255 bp and 674 bp PAX8 gene fragments was successful for all specimens analyzed regardless of storage duration. The 1039 bp beta-globin gene fragment alone failed to generate amplicons for 50% and 20% of the specimens stored for 10 and 11 years, respectively.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform DNA PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 yr
10 yr
11 yr
PCR Specific Length of gene fragment 255 bp
674 bp
1039 bp
PCR Specific Targeted nucleic acid PAX8
Beta-globin