Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Effect of platelet-associated virus on assays of HIV-1 in plasma.

Author(s): Lee TH, Stromberg RR, Henrard D, Busch MP

Publication: Science, 1993, Vol. 262, Page 1585-6

PubMed ID: 8248811 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of plasma and platelet preparation methods on HIV-1 viral load quantification. Specimens were collected from 2 HIV-1 seropositive patients. The authors do not specify what type of anticoagulant or collection tubes were used or whether fresh or frozen blood specimens were used.

   Summary of Findings:

   A higher concentration of HIV-1 RNA was found in purified platelets prepared by centrifugation of whole blood for 5 min at 250 x g, leukodepletion, and pelleting of platelets at 10,000 x g for 15 min plus washing of the pellet than in platelet free plasma prepared by centrifugation of whole blood at 200 x g for 15 min, 1000 x g for 15 min, and lastly 10,000 x g for 15 min. Platelet pellets from this last centrifugation step harbored a significant amount of HIV-1 RNA.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • AIDS/HIV-related

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Platelet-poor plasma Platelet-rich plasma Platelets
   Biospecimen Aliquots and Components	Centrifugation	Multiple durations compared Multiple speeds compared Different number of centrifugation steps compared

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