Quality assurance in analysis of cryogenically stored liver tissue specimens from the NIST National Biomonitoring Specimen Bank (NBSB).

Author(s): Mackey EA, Demiralp R, Fitzpatrick KA, Porter BJ, Wise SA, Becker PR, Greenberg RR

Publication: Sci Total Environ, 1999, Vol. 226, Page 165-76

PubMed ID: 10085566 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if the temperature (-80 or -150 degrees C) of long term specimen storage affects trace element composition. Subsets of five liver specimens snap-frozen and stored in pre-cleaned Teflon jars at -150 degrees in a liquid nitrogen vapor cooled freezer or a -80 degree C freezer for 17 years were examined.

   Summary of Findings:

   No macroscopic evidence of specimen degradation was observed among cryopreserved liver specimens stored at either temperature. Specimen trace element composition (Rb, Fe, Zn) was unaltered by 17 years of storage at either storage temperature. The following elements were not altered by prolonged storage at either temperature, although several elements were at or below the detection limit of the platform: As, Mo, Cd, Sm, La.

   Biospecimens

   • Tissue - Liver

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Atomic absorption spectroscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-80 degrees C -150 degrees C
   Storage	Storage duration	7 y 17 y

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