Effect of short-term mechanical stimulation on the salivary concentrations of vitamin C, vitamin E, total antioxidant capacity and total oxidant status.
Author(s): Lemes AL, Amadeu JK, Berrocal CS, Schussel JL, Bakkal M, Amenábar JM
Publication: Scand J Clin Lab Invest, 2018, Vol. 78, Page 556-559
PubMed ID: 30278778 PubMed Review Paper? No
Purpose of Paper
This paper investigated the effects of mechanical stimulation on saliva flow rate and vitamin C, vitamin E, total antioxidant, and total oxidant concentrations in saliva specimens.
Conclusion of Paper
Average saliva flow rate decreased after 4 min of stimulation and then remained constant for the other time-points (6, 8, and 10 min), but no statistical differences in vitamin E concentration, vitamin C concentration, total antioxidant capacity (TAC), or total oxidant status (TOS) were observed for any of the time-points analyzed.
Studies
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Study Purpose
This study investigated the effects of mechanical stimulation on saliva flow rate and vitamin C, vitamin E, total antioxidant, and total oxidant concentrations in saliva specimens. To stimulate saliva production, 40 healthy non-smokers (10 males, 30 females; 18-38 y old) chewed paraffin film. Participants were instructed not to eat, drink, or brush 1 h prior to collection between 9:30 and 11:30 am. Saliva was collected every 2 min for 10 min into plastic tubes and stored in an iced cooler. Saliva flow rate was determined immediately and then specimens were stored at -20°C for a maximum of two weeks. Specimens were centrifuged at 2600 x g for 10 min at 4°C before analysis to remove cellular and food debris. Vitamin E, vitamin C, total antioxidant capacity (TAC), and total oxidant status (TOS) were determined using spectrophotometric methods.
Summary of Findings:
Average saliva flow rate was 2.36 mL/min (± 1.09) after 2 min of stimulation and decreased to 2.04 mL/min (± 0.86) after 4 min; remaining constant for the other time-points (P=0.07) with no statistically significant difference in flow rate between males and females (data not provided). No differences in vitamin E concentration, vitamin C concentration, TAC, or TOS were observed for any of the time-points analyzed (P>0.05 for all).
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Method of fluid acquisition Multiple stimulation durations compared
Preaquisition Patient gender Female
Male