Stability of HE4 and CA125 in blood samples from patients diagnosed with ovarian cancer.
Author(s): Sandhu N, Karlsen MA, Høgdall C, Laursen IA, Christensen IJ, Høgdall EV
Publication: Scand J Clin Lab Invest, 2014, Vol. 74, Page 477-84
PubMed ID: 24724578 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of delayed centrifugation of blood and freeze-thaw cycling on measurement of human epididymis protein 4 (HE4) and carcinoma antigen 125 (CA-125) in plasma and serum from patients with ovarian cancer.
Conclusion of Paper
HE4 and CA-125 levels were not significantly different between EDTA plasma and serum specimens nor were they affected by an up to 72 h delay in centrifugation at either 4 degrees C or room temperature or up to 8 freeze-thaw cycles. While CA-125 levels were 6% lower in specimens stored at 4 degrees C than room temperature, storage temperature had no effect on levels of HE4.
Studies
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Study Purpose
The purpose of this study was to determine the effects of delayed centrifugation of blood and freeze-thaw cycling on measurement of HE4 and CA-125 in plasma and serum from patients with ovarian cancer. Blood was collected into K3EDTA and serum tubes preoperatively from 13 ovarian cancer patients. Specimens were stored at 4 or 20 degrees C for 1-72 h before centrifugation. Serum and plasma were then stored at -80 degrees C for 5 years. For freeze-thaw cycling experiments, specimens were thawed at room temperature for 1 h and then refrozen at -80 degrees C and thawed 24 h later.
Summary of Findings:
HE4 and CA-125 levels were not significantly different between EDTA plasma and serum specimens nor were they affected by an up to 72 h delay in centrifugation at 4 degrees C or room temperature or up to 8 freeze-thaw cycles. While CA-125 levels were 6% lower in specimens stored at 4 degrees C than room temperature (p=0.003), storage temperature had no effect on levels of HE4.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Glycoprotein Immunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Storage Storage temperature 4 degrees C
20 degrees C
Storage Storage duration 1 h
3 h
8 h
24 h
72 h
Storage Freeze/thaw cycling 1 cycle
2 cycles
3 cycles
4 cycles
5 cycles
6 cycles
7 cycles
8 cycles
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated