Vitamin B6 in plasma - sample stability and the reference limits.
Author(s): Panton KK, Farup PG, Sagen E, Sirum UF, Asberg A
Publication: Scand J Clin Lab Invest, 2013, Vol. 73, Page 476-9
PubMed ID: 23819643 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of storage duration and temperature and patient gender on levels of the B6 vitamer pyridoxal-5'-phosphate (p-PLP) in EDTA-plasma.
Conclusion of Paper
Generally, p-PLP was stable in EDTA-plasma for 24 h at room temperature or 4-8 degrees C, but a lower value was detected in 1 of 6 specimens after 4 h at room temperature and after 24 h at 4-8 degrees C. p-PLP concentration was 4 nmol/L higher in males than females, but the difference was not significant.
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage duration and temperature and patient gender on levels of p-PLP in EDTA-plasma. All specimens were protected from light during storage. Following experimental storage, specimens were frozen at -80 degrees C until analysis.
Summary of Findings:
Generally, p-PLP was stable in EDTA-plasma for 24 h at room temperature or 4-8 degrees C, but a lower value was detected in 1 of 6 specimens after 4 h at room temperature and after 24 h at 4-8 degrees C. p-PLP concentration was 4 nmol/L higher in males than females, but the difference was not significant.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule HPLC Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 1 h
2 h
4 h
8 h
24 h
Storage Storage temperature 4-8 degrees C
22-24 degrees C
Preaquisition Patient gender Female
Male