Heparin and EDTA anticoagulants differentially affect the plasma cytokine levels in humans.

Author(s): Patil R, Shukre S, Paranjape R, Thakar M

Publication: Scand J Clin Lab Invest, 2013, Vol. 73, Page 452-5

PubMed ID: 23772882 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of type of anticoagulant on the measurement of cytokines in plasma from healthy individuals and HIV positive patients. Plasma was stored at -80 degrees C for between 1 month and 1 year before analysis.

   Summary of Findings:

   When specimens from healthy individuals and HIV positive patients were analyzed together, significantly higher levels of tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, IL-4, granulocyte colony-stimulating factor (G-CSF), and IL-5 were measured in EDTA plasma than in heparin plasma (all p<0.05). On the other hand, significantly higher levels of macrophage inflammatory protein (MIP)-1beta, granulocyte-macrophage colony-stimulating factor (GM-CSF), monocyte chemotactic protein-1 (MCP-1), IL-6, IL-8, IL-10, and IL-17 were measured in heparin plasma than in EDTA plasma (all p<0.05). The type of anticoagulant did not affect levels of IL-7, IL-12, or IL-13. When specimens from healthy individuals and HIV positive patients were analyzed separately, the only significant difference was that IL-7 levels were higher in EDTA plasma than heparin plasma from healthy controls, but they were higher in heparin plasma than EDTA plasma from HIV patients.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • AIDS/HIV-related
   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Immunoassay
   Glycoprotein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	EDTA Heparin
   Preaquisition	Diagnosis/ patient condition	HIV positive Normal

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